biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2018-7-2-4-12bioph-231Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПРИМЕНЕНИЕ УСТРОЙСТВ ДЛЯ ПРОСТРАНСТВЕННО-РАЗРЕШЕННОЙ СПЕКТРОСКОПИИ НА ПРИМЕРЕ ДВУХСЛОЙНЫХ ФАНТОМОВ, СОДЕРЖАЩИХ МЕТАЛЛИЧЕСКИЕ НАНОЧАСТИЦЫAPPLICATION OF DEVICES FOR SPACE-RESOLVED SPECTROSCOPY ON THE EXAMPLE OF TWO-LAYER PHANTOMS CONTAINING METALLIC NANOPARTICLESХолодцоваМ. Н.KholodtsovaM. N.m.kholodtsova@gmail.comГрачевП. В.GrachevP. V.БлондельВ. C.BlondelW. C.ЗеленковП. В.ZelenkovP. V.ПотаповА. А.PotapovA. A.ЩербаковИ. А.ShcherbakovI. A.ЛощеновВ. Б.LoschenovV. B.Институт общей физики им. А.М. Прохорова РАН; Университет Лорэйн; ЦИАНФранцияProkhorov General Physics Institute of the Russian Academy of Sciences; University of Lorraine; CRANFranceИнститут общей физики им. А.М. Прохорова РАНРоссияProkhorov General Physics Institute of the Russian Academy of Sciences;Russian FederationУниверситет Лорэйн; ЦИАНФранцияUniversity of Lorraine; CRANFranceНаучно-исследовательский центр нейрохирургии им. акад. Н.Н. БурденкоРоссияN.N. Burdenko National Scientific and Practical Center for NeurosurgeryRussian FederationИнститут общей физики им. А.М. Прохорова РАНРоссияProkhorov General Physics Institute of the Russian Academy of SciencesRussian FederationИнститут общей физики им. А.М. Прохорова РАН; Национальный исследовательский ядерный университет МИФИРоссияProkhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhIRussian Federation20182506201872412Copyright © Холодцова М.Н., Грачев П.В., Блондель В.C., Зеленков П.В., Потапов А.А., Щербаков И.А., Лощенов В.Б., 20182018Холодцова М.Н., Грачев П.В., Блондель В.C., Зеленков П.В., Потапов А.А., Щербаков И.А., Лощенов В.Б.Kholodtsova M.N., Grachev P.V., Blondel W.C., Zelenkov P.V., Potapov A.A., Shcherbakov I.A., Loschenov V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/231

Исследованы возможности применения зонда для стереотаксической биопсии при флуоресцентной навигации при возбуждении 532 или 632,8 нм. Для исследования макроскопических параметров, наблюдаемых во время забора образцов при стереотаксической биопсии с помощью оптического зонда, был сделан набор полужидких, полутвердых многослойных фантомов, моделирующих границу между нормальной мозговой тканью и опухолью с фотосенсибилизатором и наночастицами золота (сферы и звезды) в различных концентрациях. Было проанализировано влияние золотых наносфер и нанозвезд, добавленных в разных концентрациях к нижнему слою фантома, на усиление флуоресценции за счет переноса энергии между наночастицами и флуоресцентными молекулами. Результаты позволяют определить границу опухоли и повысить эффективность биопсии, наблюдая характер изменения флуоресцентного сигнала при прохождении зонда через опухоль.

The abilities of the optical probe for fluorescence-guided stereotactic biopsy were investigated by means of a multifiber probe under 532 or 632.8 nm excitation. The set of multilayered phantoms representing a border between normal brain-tissue and a tumor with photosensitizer and gold nanoparticles (spheres or stars) in different concentrations were made to investigate the macroscopic parameters observed during the neurosurgical biopsy sample collection by means of the optical probe. These investigantions will allow to define the border of a brain tumor and control the type of tissue being collected by a stereotactic cannula during surgery. The impact of gold nanospheres and gold nanostars added in different concentrations to the bottom-layer of phantom was analyzed by fluorescence quenching or enhancement due to energy transfer between nanoparticles and fluorescent molecules. The results allow determining the tumor border and to make the biopsy uptake more efficient by observing the character of signal change while penetrating a tumor and while going out of it.

плазмонный резонанснаночастицы металловглубина оптического зондированияглубина зондирования флуоресценцииглиальные опухолибиологическая тканьмногослойная тканьпространственно разрешенная спектроскопия5-аминолевулиновая кислотапротопорфирин IXстереотактическая биопсияфлуоресцентная спектроскопияplasmon resonancemetal nanoparticlesoptical probing depthfluorescence probing depthglial tumoursbiological tissuemulti-layered tissuespatially resolved spectroscopy5-aminolevulinic acidprotoporphyrin IXstereotactic biopsyfluorescence spectroscopyReferencesChampion J., Katare Y., Mitragotri S. Particle shape: A new design parameter for micro- and nanoscale drug delivery carriers // Jour- nal of Controlled Release. – 2007. – Vol. 121. – P. 3–9.Champion J., Katare Y., Mitragotri S. Particle shape: A new designparameter for micro- and nanoscale drug delivery carriers, Journal of Controlled Release, 2007, Vol. 121, pp. 3–9.Weibo C., Ting G., Hao H., Sun J. Applications of gold nanopar- ticles in cancer and nanotechnology // Nanotechnology, Science and Applications. – 2008. – № 1. – P. 17–32.Weibo C., Ting G., Hao H., Sun J. Applications of gold nanopar- ticles in cancer and nanotechnology, Nanotechnology, Science and Applications, 2008, No. 1, pp. 17–32.Bohren C., Huffman D. Absorption and Scattering of Light by Small Particles. – Wiley, VCH Verlag GmbH, 1998. – 545 p.Bohren C., Huffman D. Absorption and Scattering of Light by Small Particles. Wiley, VCH Verlag GmbH, 1998. 545 p.Cihan C., Arifler D. Influence of phase function on modeled opti- cal response of nanoparticle-labeled epithelial tissues // Journal of Biomedical Optics. – 2011. – Vol. 16(8). – 085002.Cihan C., Arifler D. Influence of phase function on modeled opti- cal response of nanoparticle-labeled epithelial tissues, Journal of Biomedical Optics, 2011, Vol. 16(8), 085002.Потапов А.А., Гаврилов А.Г., Горяйнов С.А. и др. Способ проведения интраоперационной комбинированной спектроскопической диагностики опухолей головного и спинного мозга Simultanious parenchymal organ biopsy and spectroscopic inspection device. – Патент РФ №2497558, 2013. – 16 с.Potapov A.A., Gavrilov A.G., Goryaynov S.A., Zelenkov P.V., Gol’bin D.A., Savel’eva T.A. et al. Sposob provedeniya intraoperatsionnoy kom- binirovannoy spektroskopicheskoy diagnostiki opukholey golovnogo i spinnogo mozga [Simultanious parenchymal organ biopsy and spectroscopic inspection device]. Patent RF no. 2497558, 2013. 16 p.Markwardt N.A., Haj-Hosseini N., Hollnburger B., et al. 405 nm versus 633 nm for protoporphyrin IX excitation in fluorescence- guided stereotactic biopsy of brain tumors // Journal of Biopho- tonics. – 2015. – Vol. 9(9). – P. 901–912.Markwardt N. A., Haj-Hosseini N., Hollnburger B., Stepp, H., Zelen- kov, P., Ruhm A. 405 nm versus 633 nm for protoporphyrin IX exci- tation in fluorescence-guided stereotactic biopsy of brain tumors, Journal of Biophotonics, 2015, Vol. 9(9), pp. 901–912.Blondel W.C., Ghribi M., Leroux A., et al. Spectral features selec- tion and classification for bimodal optical spectroscopy applied to bladder cancer in vivo diagnosis // IEEE Transactions on Biomedi- cal Engineering. – 2014. – Vol 61(1). – P. 207–216.Blondel W.C., Ghribi M., Leroux A., Pery E., Tindel S. Spectral fea- tures selection and classification for bimodal optical spectros- copy applied to bladder cancer in vivo diagnosis, IEEE Transactions on Biomedical Engineering, 2014, Vol 61(1), pp. 207–216.Rick K., Sroka R., Stepp H., Kriegmair M., et al. Pharmacokinetics of 5-aminolevulinic acid-induced protoporphyrin ix in skin and blood // Journal of Photochemistry and Photobiology B-Biology. – 1997. – Vol. 40(3). – P. 313–319.Rick K., Sroka R., Stepp H., Kriegmair M., Huber R.M., Jacob K., Baumgartner R. Pharmacokinetics of 5-aminolevulinic acid- induced protoporphyrin ix in skin and blood, Journal of Photo- chemistry and Photobiology B-Biology, 1997, Vol. 40(3), pp. 313–319.Wuithschick M., Birnbaum A., Witte S., et al. Turkevich in new robes: Key questions answered for the most common gold nanoparticle synthesis // ACS Nano. – 2015. – Vol. 9(7). – P. 7052–7071.Wuithschick M., Birnbaum A., Witte S., Sztucki M., Vainio U., Pinna N., Rademann K., Emmerling F., Kraehnert R., Polte J. Turkevich in new robes: Key questions answered for the most common gold nanoparticle synthesis, ACS Nano, 2015, Vol. 9(7), pp. 7052–7071.Hrelescu C., Sau T.K. Selective excitation of individual plasmonic hotspots at the tips of single gold nanostars // Nano Letters. –2011. – Vol. 11. – P. 402–407.Hrelescu C., Sau T.K. Selective excitation of individual plasmonic hotspots at the tips of single gold nanostars, Nano Letters, 2011, Vol. 11, pp. 402–407.Scaffard L., Tocho J. Size dependence of refractive index of gold nanoparticles // Nanotechnology. – 2006. – Vol. 17. – P. 1309–1315.Scaffard L., Tocho J. Size dependence of refractive index of gold nanoparticles, Nanotechnology, 2006, Vol. 17, pp. 1309–1315.Kholodtsova M., Samsonova I., Blondel W., Loschenov V. Metal nanoparticles of different shapes influence on optical properties of multilayered biological tissues // Proceedings of SPIE. -2015. – Vol. 9542. – 954205.Kholodtsova M., Samsonova I., Blondel W., Loschenov V. Metal nanoparticles of different shapes influence on optical properties of multilayered biological tissues, Proceedings of SPIE, 2015, Vol. 9542, 954205.Kang K., Wang J., Jasinski J., Achilefu S. Fluorescence manipulation by gold nanoparticles: From complete quenching to extensive enhance- ment // Journal of Nanobiotechnology. – 2011. – Vol. 9. – P. 16.Kang K., Wang J., Jasinski J., Achilefu S. Fluorescence manipulation by gold nanoparticles: From complete quenching to extensive enhancement, Journal of Nanobiotechnology, 2011, Vol. 9, p. 16.Ninni P.D., Martelli F., Zaccanti G. The use of India Ink in tissue-simu- lating phantoms // Optics Express. – 2010. – Vol. 18(26). – P. 26854–26865.Ninni P.D., Martelli F., Zaccanti G. The use of India Ink in tissue-simu- lating phantoms, Optics Express, 2010, Vol. 18(26), pp. 26854–26865.Ninni P.D., Martelli, F., Zaccanti G. Intralipid: towards a diffusive ref- erence standard for optical tissue phantoms // Physics in Medicine and Biology. – 2011. – Vol. 56. – P. 21–28.Ninni P.D., Martelli, F., Zaccanti G. Intralipid: towards a diffusive ref- erence standard for optical tissue phantoms, Physics in Medicine and Biology, 2011, Vol. 56, pp. 21–28.Flock S.T., Jacques S.L., Wilson B.C., et al. Optical properties of intralipid: A phantom medium for light propagation studies // Lasers in Surgery and Medicine. – 1992. – Vol. 12(5) – P.510–519.Flock S.T., Jacques S.L., Wilson B.C., Star W.M., van Gemert M.J. Opti- cal properties of intralipid: A phantom medium for light propagation studies, Lasers in Surgery and Medicine, 1992, Vol. 12(5), pp. 510–519.Yaroslavsky N., Schulze P., Yaroslavsky I.V., et al. Optical properties of selected native and coagulated human brain tissues in vitro in the visible and near infrared spectral range // Physics in Medicine and Biology. – 2002. – Vol. 47(12). – P. 2059–2073.Yaroslavsky N., Schulze P., Yaroslavsky I.V., Schober R., Ulrich F., Schwar- zmaier H.J. Optical properties of selected native and coagulated human brain tissues in vitro in the visible and near infrared spectral range, Physics in Medicine and Biology, 2002, Vol. 47(12), pp. 2059–2073.Bashkatov, A., Genina, E., Tuchin, V., Optical properties of skin, subcutaneous, and muscle tissues: a review // Journal of Innova- tive Optical Health Science. – 2011. – Vol. 04(01). – P.9–38.Bashkatov, A., Genina, E., Tuchin, V., Optical properties of skin, subcutaneous, and muscle tissues: a review, Journal of Innovative Optical Health Science, 2011, Vol. 04(01), pp. 9–38.Kholodtsova M.N., Grachev P.V., Savelieva T.A., et al. Scattered and fluorescent photon track reconstruction in a biological tissue // International Journal of Photoenergy. – 2014. – 517510.Kholodtsova M.N., Grachev P.V., Savelieva T.A., Kalyagina N.A., Blondel W., Loschenov V.B. Scattered and fluorescent photon track reconstruction in a biological tissue, International Journal of Photoenergy, 2014, 517510.Haiss W., Thanh N., Aveyard J., Fernig, D. Determination of size and concentration of gold nanoparticles from uv-vis spectra // Ana- lytical Chemistry. – 2007. – Vol. 79. – P.4215–4221.Haiss W., Thanh N., Aveyard J., Fernig, D. Determination of size and concentration of gold nanoparticles from uv-vis spectra, Analytical Chemistry, 2007, Vol. 79, pp.4215–4221.

The authors declare that there are no conflicts of interest present.