biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2018-7-3-4-20bioph-248Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESАктивация наночастиц фталоцианина алюминия для локальной флуоресцентной спектроскопии в стоматологииAluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistryЗолотареваЮ. О.ZolotarevaJ. O.JOKuznetsova@mail.ruФарраховаД. С.FarrakhovaD. S.КуприяноваЕ. Н.KupriyanovaE. N.ЛощеновВ. Б.LoschenovV. B.Национальный исследовательский ядерный университет МИФИРоссияNational Research Nuclear University MEPhIRussian FederationНациональный исследовательский ядерный университет МИФИ; Институт общей физики им. А.М. Прохорова Российской Академии Наук; ООО «Дентоспек»РоссияNational Research Nuclear University MEPhI; Prokhorov General Physics Institute of the Russian Academy of Sciences; Dentospek LLCRussian FederationАУЗ «Стоматологическая поликлиника №11 ДЗМ»РоссияSAHI «Dental Clinic No. 11 of the Moscow City Health Department»Russian Federation20181010201873420Copyright © Золотарева Ю.О., Фаррахова Д.С., Куприянова Е.Н., Лощенов В.Б., 20182018Золотарева Ю.О., Фаррахова Д.С., Куприянова Е.Н., Лощенов В.Б.Zolotareva J.O., Farrakhova D.S., Kupriyanova E.N., Loschenov V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/248

Для выявления микротрещин эмали и очагов скопления патогенной микрофлоры, которые могут стать причиной развития кариеса, в качестве маркера используется фталоцианин алюминия (AlPc) в виде наночастиц. В коллоидном растворе наночастицы не обладают собственной флуоресценцией, в отличие от молекулярной формы. Для перевода частицы в молекулярную форму необходимо присутствие растворителя или специфического окружения (бактерии, макрофаги и др.). Поэтому гидрофобные наночастицы фталоцианина алюминия (nAlPc) могут выступать в качестве маркера для обнаружения скрытых очагов скопления патогенной микрофлоры во время проведения флуоресцентной диагностики. Для сокращения времени диагностики и увеличения эффективности в качестве дополнительных активаторов nAlPc могут быть использованы биологически совместимые поверхностно-активные вещества (ПАВ).

Для проведения локальной флуоресцентной спектроскопии микротрещин эмали и очагов скопления патогенной микрофлоры на поверхности эмали была приготовлена модельная смесь, содержащая ПАВ, вспомогательные компоненты и коллоида nAlPc в концентрации 10 мг/л.

Исследования по взаимодействию модельной смеси с nAlPc и протеланом с эмалью зубов ex vivo показали перспективность использования этого ПАВ для дополнительной активации наночастиц, что позволяет проводить локальную флуоресцентную спектроскопию поверхности эмали зубов через 3 мин после нанесения. Также статистическая обработка результатов показала эффективность использования модельной смеси для локальной флуоресцентной спектроскопии поверхности эмали для выявления микротрещин эмали и очагов скопления патогенной микрофлоры, которая может привести к развитию кариесогенного процесса.

Early diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. In

order to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker. In a colloidal solution, the nanoparticles do not fluoresce, unlike their molecular form. To convert the particle into its molecular form, it is necessary to have a solvent or specific environment (bacteria, macrophages, etc.). That is why the hydrophobic nanoparticles of aluminum phthalocyanine (nAlPc) can act as markers for detecting hidden pathogenic microflora during fluorescent diagnostics. Further reduction of the diagnosis time and increase the efficiency can be achieved by using biologically compatible surfactants as additional activators of nAlPc.

In order to carry out local fluorescence spectroscopy of enamel microcracks and pathogenic microflora foci on the enamel surface, a model compound containing surfactants, auxiliary components and nAlPc colloid at a concentration of 10 mg/l was prepared.

Studies on the interaction of the model compound with nAlPc and Protelan MST-35 with tooth enamel ex vivo have shown this surfactant to be a promising auxiliary activator of the nanoparticles, allowing conducting local fluorescence spectroscopy of the tooth enamel surface 3 min after application. In addition, statistical processing of the results showed the effectiveness of using the model compound for local fluorescence spectroscopy of the enamel surface in order to detect the enamel microcracks and the pathogenic microflora accumulation foci that can lead to the development of a cariogenic process.

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The authors declare that there are no conflicts of interest present.