biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2019-8-1-38-45bioph-292Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЛазерная флуоресцентная спектроскопия и оптическая тканевая оксиметрия в диагностике фиброза кожиLaser fluorescence spectroscopy and optical tissue oximetry in the diagnosis of skin fibrosisЧурсиноваЮ. В.ChursinovaYu. V.yu.chursinova@monikiweb.ruКуликовД. А.KulikovD. A.РогаткинД. А.RogatkinD. A.РазницынаИ. А.RaznitsynaI. A.МосальскаяД. В.MosalskayaD. V.БобровМ. А.BobrovM. A.ПетрицкаяЕ. Н.PetritskayaE. N.МолочковА. В.MolochkovA. V.Государственное бюджетное учреждение здравоохранения Московской области «Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского» (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского), МоскваРоссияMoscow Regional Research and Clinical Institute (“MONIKI”), MoscowRussian FederationГосударственное бюджетное учреждение здравоохранения Московской области «Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского» (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского), Москва; Национальный исследовательский ядерный университет МИФИ, МоскваРоссияMoscow Regional Research and Clinical Institute (“MONIKI”), Moscow; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), MoscowRussian Federation201927032019813845Copyright © Чурсинова Ю.В., Куликов Д.А., Рогаткин Д.А., Разницына И.А., Мосальская Д.В., Бобров М.А., Петрицкая Е.Н., Молочков А.В., 20192019Чурсинова Ю.В., Куликов Д.А., Рогаткин Д.А., Разницына И.А., Мосальская Д.В., Бобров М.А., Петрицкая Е.Н., Молочков А.В.Chursinova Y.V., Kulikov D.A., Rogatkin D.A., Raznitsyna I.A., Mosalskaya D.V., Bobrov M.A., Petritskaya E.N., Molochkov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/292

В современной медицинской практике нет эффективных мер борьбы с фиброзом. Одна из причин – поздняя диагностика, связанная с отсутствием доступных клинических биомаркеров и эффективных методов неинвазивного обнаружения этого процесса. Фиброзирующие заболевания кожи характеризуются фиброзом дермы, подлежащих тканей и представлены широким спектром нозологий. Наибольший интерес для изучения представляют склеродермия и рубцы кожи. На экспериментальной модели методами лазерной флуоресцентной спектроскопии и оптической тканевой оксиметрии изучены изменения кожи в рамках развития блеомицин-индуцированного фиброза. Выявлен достоверный рост показателей эндогенной флуоресценции порфиринов на 7 и на 21 сут, вызванный воспалением и гипоксией. Зафиксированы повышение интенсивности эндогенной флуоресценции коллагена и снижение показателей удельного потребления кислорода на 21 сут исследования, связанные с избыточным накоплением межклеточного матрикса. Синхронные измерения флуоресценции коллагена и удельного потребления кислорода позволили провести корреляцию с фазами фиброгенного ответа, описанного морфологически. Полученные результаты позволяют судить о выраженности воспаления и гипоксии в процессе развития фиброза. Объективный и количественный характер регистрируемых параметров дает возможность разработки критериев для диагностики фаз развития фиброза.

There are currently no effective measures to combat fibrosis in modern medical practice. One of the reasons for that is the late diagnosis associated with the lack of available clinical biomarkers and effective methods of non-invasive detection of the process. Fibrosis of the skin is characterized by fibrosis of the dermis, underlying tissues and is represented by a wide range of nosologies. Scleroderma and scars are of the greatest interest for the study. Skin changes in the development of bleomycin-induced fibrosis was studied in the experimental model using laser fluorescence spectroscopy and optical tissue oximetry. A significant increase in the rates of endogenous fluorescence of porphyrins, caused by inflammation and hypoxia, was detected at 7 and 21 days. An increased intensity of endogenous collagen fluorescence and a decreased specific oxygen uptake due to excess accumulation of the extracellular matrix were recorded on the 21st day after bleomycin treatment. Synchronous measurements of the collagen fluorescence and the specific oxygen uptake allowed to correlate the obtained data and the phases of the fibrogenic response described morphologically. The results allow to judge the severity of inflammation and hypoxia in the process of the fibrosis development. The objective and quantitative nature of the recorded parameters makes it possible to develop criteria for diagnosing the phases of fibrosis development.

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The authors declare that there are no conflicts of interest present.