biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2019-8-2-4-13bioph-312Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭффективность фотодинамического воздействия в сочетании с озоном и хлорофиллом из листьев Dracaena angustifolia на биопленки Staphylicoccus aureusPhotodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leavesAstutiS. D.AstutiS. D.suryanidyah@fst.unair.ac.idDrantantiyasN. D.DrantantiyasN. D.PutraA. P.PutraA. P.PuspitaP. S.PuspitaP. S.SyahromA.SyahromA.SuhariningsihS.SuhariningsihS.Университет АирлангаИндонезияAirlangga UniversityIndonesiaТехнологический институт СуматрыИндонезияSumatera Institute of TechnologyIndonesiaТехнологический Институт Сепулух НопемберИндонезияSepuluh Nopember Institute of TechnologyIndonesiaТехнологический университет МалайзииМалайзияUniversiti Teknologi MalaysiaMalaysia20191007201982413Copyright © Astuti S.D., Drantantiyas N.D., Putra A.P., Puspita P.S., Syahrom A., Suhariningsih S., 20192019Astuti S.D., Drantantiyas N.D., Putra A.P., Puspita P.S., Syahrom A., Suhariningsih S.Astuti S.D., Drantantiyas N.D., Putra A.P., Puspita P.S., Syahrom A., Suhariningsih S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/312

Photodynamic inactivation is an effective treatment that uses light irradiation, photosensitizer and oxygen. The aim of this study was to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll (Chlo). The chlorophyll was extracted from leave of Dracaena angustifolia. To determine the antibacterial effect of S. aureus biofilm treatments, samples were separated into Chlo, Laser, Chlo+Laser, Ozone, Ozone+Laser, Chlo+Ozone+Laser categories. The data were analyzed using ANOVA test. The result of this study showed that Chlo+Ozone+Laser combine treatment at 20 s exposure of ozone with 4 min of irradiation time lead to 80.26 % reduction of biofilm activity, which was the highest efficacy of all the treatment groups. The combination of laser, chlorophyll and lower ozone concentration increases the effectiveness of photodynamic inactivation.

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The authors declare that there are no conflicts of interest present.