biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2020-9-2-10-17bioph-422Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESУзкополосная фототерапия и препарат натриевой соли синтетического дипептида гамма-D-глутамил-D-триптофана в качестве комбинированного метода лечения псориаза: комплексная и сравнительная оценкаNarrow-band phototherapy and sodium salt of the synthetic diamide gamma-D-glutamyl-D-tryptophan as a combined method of psoriasis treatment: comprehensive and comparative evaluationНовиковЮ. А.NovikovY. A.

Омск

Omsk

ОхлопковВ. А.OkhlopkovV. A.

Москва

Moscow

ТрошинаД. С.TroshinaD. S.

Омск

Omsk

kastaliya@mail.ruМозговойС. И.MozgovoyS. I.

Омск

Omsk

ПолещукЕ. И.PoleshchukE. I.

Омск

Omsk

ПравдинаО. В.PravdinaO. V.

Омск

Omsk

Омский государственный медицинский университетРоссияOmsk state medical University of Health of the Russian FederationRussian FederationФедеральный научно-клинический центр реаниматологии и реабилитологииРоссияFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologyRussian Federation202024072020921017Copyright © Новиков Ю.А., Охлопков В.А., Трошина Д.С., Мозговой С.И., Полещук Е.И., Правдина О.В., 20202020Новиков Ю.А., Охлопков В.А., Трошина Д.С., Мозговой С.И., Полещук Е.И., Правдина О.В.Novikov Y.A., Okhlopkov V.A., Troshina D.S., Mozgovoy S.I., Poleshchuk E.I., Pravdina O.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/422

В работе представлены результаты сравнительного исследования различных иммуносупрессивных методов лечения вульгарного псориаза среднетяжёлой степени (PASI от 10 до 20). В качестве оцениваемых параметров использовали индекс PASI, уровни экспрессии маркера апоптоза р53 и экспрессии маркера Т-регуляторных клеток Foxp3. В исследование были включены 96 пациентов в возрасте от 18 до 60 лет, разделённые методом рандомизации на три группы. Пациенты в 1-й группе (n=32) получали узкополосную фототерапию по 4-дневной схеме. Использовали прибор «Waldmann UV-7002K» и лампы F79/120W-TL01, генерирующие излучение с максимальной эмиссией на длине волны 311 нм. Начинали с дозы 0,05 – 0,1 Дж/см2, повышая в последующем на 0,05 – 0,1 Дж/см2 каждую процедуру, при отсутствии эритемы. Всего пациенты получали от 12 до 15 процедур с курсовой дозой облучения 4,7 –7,65 Дж/см2. Во 2-й группе (n=32) пациентам проводили терапию натриевой солью синтетического дипептида гамма-D-глутамил-D-триптофана. Пациентам 3-й группы (n=32) проводили комбинированную терапию, включающую в себя сочетание двух вышеописанных методик. В результате лечения наибольшее снижение индекса PASI, а также уровня экспрессии маркера Т-регуляторных клеток Foxp3 наблюдали среди пациентов, получивших комбинированную схему. Полученные данные свидетельствуют о высо- кой терапевтической эффективности узкополосной фототерапии в комбинации с препаратом натриевой соли синтетического дипептида гамма- D-глутамил-D-триптофана. Также было отмечено, что иммуногистохимический маркер р53, отражающий клеточное обновление кератиноцитов псориатической папулы, является информативным молекулярно-клеточным показателем эффективности узкополосной фототерапии.

The paper presents the results of a comparative study of various immunosuppressive treatment methods of medium severity vulgar psoriasis (PASI from 10 to 20). The parameters used were PASI indices, p53 apoptosis marker expression indices, and expression indices of T-regulatory cell marker Foxp3. The study involved 96 patients, from 18 to 60 years old, divided by randomization into three groups. Patients in the 1st group (n = 32) received narrow-band phototherapy according to a 4-day regimen. We used the Waldmann UV-7002K instrument and F79/120W-TL01 lamps generating radiation with a maximum emission at a wavelength of 311 nm. We started with a dose of 0.05–0.1 J/cm2, subsequently increased by 0.05–0.1 J/cm2 for each procedure, in the absence of erythema. In total, patients received 12–15 procedures, with a course dose of 4.7–7.65 J/cm2. In the 2nd group (n = 32), patients were treated with the sodium salt of the synthetic gamma-D-glutamyl-D tryptophan dipeptide. Patients of the 3rd group (n = 32) underwent combination therapy, which included both methods described above. As a result of treatment, the greatest decrease in the PASI index, as well as the level of expression of the marker of Foxp3 T-regulatory cells, was observed among patients who received the combined regimen. The data obtained indicate the high therapeutic efficacy of narrow-band phototherapy in combination with the sodium salt of the synthetic gamma-D glutamyl-D tryptophan synthetic dipeptide. It was also noted that the immunohistochemical marker p53, reflecting the cellular renewal of psoriatic papule keratinocytes, is an informative molecular-cellular indicator of the effectiveness of narrow-band phototherapy.

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The authors declare that there are no conflicts of interest present.