biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2020-9-2-45-54bioph-427Research ArticleКЛИНИЧЕСКИЕ НАБЛЮДЕНИЯCASE-REPORTSЛечебный патоморфоз в тканях злокачественной глиомы после фотодинамической терапии с хлорином е6 (сообщение о двух клинических случаях)Therapeutic pathomorphosis in malignant glioma tissues after photodynamic therapy with сhlorin e6 (reports of two clinical cases)РындаА. Ю.RyndaA. Yu.

Санкт-Петербург

Saint-Petersburg

artemii.rynda@mail.ruРостовцевД. М.RostovtsevD. M.

Санкт-Петербург

Saint-Petersburg

ОлюшинВ. Е.OlyushinV. E.

Санкт-Петербург

Saint-Petersburg

ЗабродскаяЮ. М.ZabrodskayaYu. M.

Санкт-Петербург

Saint-Petersburg

Российский нейрохирургический институт имени профессора А.Л. Поленова - филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»РоссияRussian Neurosurgical Institute named after Professor A.L. Polenov - a branch of «The National Medical Research Center named after V.A. Almazov»Russian Federation202024072020924554Copyright © Рында А.Ю., Ростовцев Д.М., Олюшин В.Е., Забродская Ю.М., 20202020Рында А.Ю., Ростовцев Д.М., Олюшин В.Е., Забродская Ю.М.Rynda A.Y., Rostovtsev D.M., Olyushin V.E., Zabrodskaya Y.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/427

Фотодинамическая терапия (ФДТ) в последние годы все более внедряется в хирургическую практику лечения злокачественных новообразований. В данной публикации авторами показано появление лечебного патоморфоза in vivo в клетках злокачественной глиомы человека после интраоперационной ФДТ. В образцах тканей, полученных через 10–14 дней после ФДТ, выявлены ядерные и цитоплазматические признаки, указывающие на апоптоз, некроз и аутофагию. Обнаружено снижение пролиферативной активности глиальных опухолевых клеток, увеличение числа случаев их гибели. По данным иммуногистохимии отмечено уменьшение экспрессии маркера клеточной пролиферации Ki-67 и снижение содержания белка транскрипционного фактора p53.

In recent years, photodynamic therapy (PDT) has been increasingly introduced into the surgical practice of treating malignant neoplasms. In this publication, the authors show the appearance of therapeutic pathomorphosis in vivo in human malignant glioma cells after intraoperative photodynamic therapy. Tissue samples obtained 10–14 days after PDT revealed nuclear and cytoplasmic signs indicating apoptosis, necrosis, and autophagy. A decrease in the proliferative activity of glial tumor cells and their higher death count were detected.. Immunohistochemical analysis shows decreases expression of Ki-67 cell proliferation marker and decreased amount of transcription factor protein p53.

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The authors declare that there are no conflicts of interest present.