biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2020-9-4-4-14bioph-453Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭффективность фотодинамической инактивации бактериальных биопленок с использованием экстракта куркумина, нанодоксициклина и лазерного диодаEffectiveness of bacterial biofilms photodynamic inactivation mediated by curcumin extract, nanodoxycycline and laser diodeAstutiS. D.AstutiS. D.

Сурабая, Восточная Ява

Surabaya, East Java

suryanidyah@fst.unair.ac.idMahmudA. F.MahmudA. F.

Сурабая, Восточная Ява

Surabaya, East Java

PutraA. P.PutraA. P.

Сурабая, Восточная Ява

Surabaya, East Java

SetiawatieE. M.SetiawatieE. M.

Сурабая, Восточная Ява

Surabaya, East Java

ArifiantoD.ArifiantoD.

Сурабая, Восточная Ява

Surabaya, East Java

Университет АирланггаИндонезияUniversitas AirlanggaIndonesia20200402202194414Copyright © Astuti S.D., Mahmud A.F., Putra A.P., Setiawatie E.M., Arifianto D., 20212021Astuti S.D., Mahmud A.F., Putra A.P., Setiawatie E.M., Arifianto D.Astuti S.D., Mahmud A.F., Putra A.P., Setiawatie E.M., Arifianto D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/453

Биопленки обладают более высоким уровнем устойчивости к антибиотикам по сравнению с бактериями, поэтому необходима разработка новых подходов к лечению инфекционных заболевания, вызванных бактериальными биопленками. Одним из возможных методов лечения таких заболеваний является фотодинамическая терапия (ФДТ). В качестве фотосенсибилизаторов применяли куркумин и антибиотик нанодоксициклин. Провели сравнительное изучение эффективности фотодинамической терапии инфекций, в патогенезе которых участвовали Aggregatibacter actinomycetemcomitans, вызывающие пародонтит, с использованием двух указанных фотосенсибилизаторов. Биопленку A. actinomycetemcomitans выращивали на 96-луночном микропланшете в течение 72 ч инкубации. Образцы были разделены на три группы. В первой группе проводили обработку биопленок диодным лазером, во второй – 0,5%-ым экстрактом куркумы и диодным лазером, в третьей – 0,01%-ым раствором нанодоксициклина и и диодным лазером. Время воздействия составляло 30, 60, 90, 120 и 150 сек. Полученные данные были проанализированы с использованием теста ANOVA. Результаты анализа данных показали, что эффективность воздействия на биопленки значительно отличалась в группах с облучением диодным лазером, облучением диодным лазером с куркумином и облучением диодным лазером с нанодоксициклином. Режимы облучения диодным лазером при различных плотностях энергии 4,15; 8,28; 12,44; 16,59; и 20,73 Дж/см2 не показали существенного воздействия на бактериальную биопленку. Облучение диодным лазером (20,73 Дж/см2, время облучения 150 сек) привело к наибольшему уменьшению биопленки на 14,94%, облучение диодным лазером с куркумином – на 47,82%, облучение диодным лазером с нанодоксициклином – на 53,76%. Таким образом, ФДТ с использованием диодного лазера в сочетании с экзогенными фотосенсибилизаторами куркумином и нанодоксициклином показали свою эффективность в отношении бактериальных биопленок.

Biofilms have higher levels of antibiotic resistance compared to bacteria, so the alternatives are needed as therapy for diseases caused by biofilm infections. Photodynamic Therapy (PDT) has the advantage of being a safe alternative that involves molecular-level photochemical reactions. The use of different types of exogenous photosensitizers (PS) was done to compare their effectiveness. Turmeric extract containing curcumin has good effectiveness in PDT, whereas nanodoxycycline as an antibiotic has a fairly broad absorption spectrum and is effective as PS. The purpose of this study is to compare the effectiveness of photodynamic therapy on infections by Aggregatibacter actinomycetemcomitans causing periodontitis using exogenous organic and non-organic photosensitisers (PS). The A. actinomycetemcomitans biofilm had been grown on 96-well microplate for 72 hours incubation time. The samples were divided into three groups, treated with Laser diode, Laser + Turmeric Extract 0.5%, and Laser + Nanodoxycycline 0.1%. Treatment was done with a variety of exposure times: 30, 60, 90, 120, and 150 seconds. The data were analyzed using ANOVA test. The results of data analysis showed that diode laser irradiation treatment with endogenous porphyrin, diode laser with Curcumin and diode laser with nanodoxycycline produced significantly different biofilm reductions. Treatment with diode laser irradiation at various energy densities (4.15, 8.28, 12.44, 16.59, and 20.73 J/cm2) showed no significant difference in reducing bacterial biofilm. The treatment with diode and curcumin, and the treatment with diode laser irradiation and nanodoxycyclin showed a significant difference. Diode laser irradiation of 20.73 J/cm2 with irradiation time of 150 seconds resulted in the greatest reduction of biofilm 14.94%, diode laser irradiation + Curcumin 47.82%, and diode laser irradiation + nanodoxycyclin 53.76%. Therefore, PDT using a diode laser combined with exogenous PS extract of curcumin and nanodoxycycline is more effective to reduce bacterial biofilms.

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The authors declare that there are no conflicts of interest present.