biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2022-11-4-4-10bioph-562Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭффективность in vitro инактивации бактерий Bacillus subtilis и Escherichia coli в стерилизаторах с использованием облучения в фиолетовой областиEffectiveness of purple led for inactivation of Bacillus subtilis and Escherichia coli bacteria in in vitro sterilizersYaqubiA. K.YaqubiA. K.

Surabaya

Surabaya

AstutiS. D.AstutiS. D.

Surabaya

Surabaya

suryanidyah@fst.unair.ac.idPermatasariP.A.D.PermatasariP.A.D.

Surabaya

Surabaya

KomariyahN.KomariyahN.

Surabaya

Surabaya

EndarkoE.EndarkoE.

Surabaya

Surabaya

ZaidanA. H.ZaidanA. H.

Surabaya

Surabaya

Airlangga UniversityИндонезияAirlangga UniversityIndonesiaSepuluh Nopember Institute of TechnologyИндонезияSepuluh Nopember Institute of TechnologyIndonesia202230012023114410Copyright © Yaqubi A.K., Astuti S.D., Permatasari P., Komariyah N., Endarko E., Zaidan A.H., 20232023Yaqubi A.K., Astuti S.D., Permatasari P., Komariyah N., Endarko E., Zaidan A.H.Yaqubi A.K., Astuti S.D., Permatasari P., Komariyah N., Endarko E., Zaidan A.H.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/562

Инактивация бактерий может быть выполнена с использованием метода, называемого фотодинамической инактивацией, в основе которого лежит активация фотосенсибилизатора светом определенного спектра. Целью данного исследования является определение эффективности светодиодов с излучением в фиолетовой области спектра для фотоинактивации бактерий Bacillus subtilis и Escherichia coli, а также определение оптимальной плотности энергии воздействия. При облучении были использованы два изменяемых параметра. Первый параметр – это расстояние от источника облучения до облучаемой поверхности (3 см, 6 см, 9 см и 12 см). Второй параметр – время облучения (30, 60, 90 и 120 мин). Для подсчета количества колоний использовали метод общего подсчета чашек (Total Plate Count). При анализе данных использовали статистические тесты, а именно тест One Way Anova (дисперсионный анализ). Результаты этого исследования показали, что светодиодное излучение в фиолетовой области спектра с длиной волны 395 нм вызывало снижение log КОЕ/мл бактерий Bacillus subtilis и Escherichia coli. Воздействие на бактерии Bacillus subtilis показало более высокий процент смертности, чем для бактерий Escherichia coli. Лучшие результаты были получены при расстоянии до источника облучения 3 см, плотности энергии 524 Дж/см2, и времени воздействия светодиода 120 мин. В этом режиме было инактивировано 98,5% бактерий Bacillus subtilis и 94,3% бактерий Escherichia coli.

Bacteria are inactivated using a technique called photodynamic inactivation, which combines light with a photosensitizer with the right spectrum. The objective of this study is to ascertain the e­ciency of purple LEDs for photoinactivating Bacillus subtilis and Escherichia coli bacteria as well as the ideal purple LED exposure energy density. This study technique involves exposing bacteria to purple LED radiation. Two elements of variation are used during irradiation. The first variation is the illumination variation at distances of 3 cm, 6 cm, 9 cm, and 12 cm. The second variation involves changing the amount of radiation for 30, 60, 90, and 120 minutes. The Total Plate Count (TPC) method was used to count the number of colonies. Statistical tests were utilized in data analysis, namely the One Way Anova test (analysis of variance). The results of this study indicated that 395 nm purple LED irradiation caused a decrease in Log CFU/mL of Bacillus subtilis and Escherichia coli bacteria. Inactivation of Bacillus subtilis bacteria showed a higher mortality percentage than Escherichia coli bacteria. Changes in other irradiation distances also showed a higher percentage of death for Bacillus subtilis bacteria than Escherichia coli bacteria. The highest percentage of death was 98.5% for Bacillus subtilis bacteria and 94.3% for Escherichia coli bacteria at position C with an irradiation distance of 3 cm and an energy density of 524 J/cm2 with an LED exposure time of 120 minutes. This shows that the percentage of death of bacteria Bacillus subtilis and Escherichia coli increased with increasing doses of LED energy with the greatest percentage of death in Gram-positive bacteria Bacillus subtilis.

безопасностьфотодинамическая инактивацияфиолетовый светодиодBacillus subtilisEscherichia colihealth securityphotodynamic inactivationpurple LEDBacillus subtilisEscherichia coliReferencesAstuti S. D. et al. Antimicrobial photodynamic effects of polychromatic light activated by magnetic fields to bacterial viability, Journal of International Dental and Medical Research, 2017, vol. 10(1), pp. 111-117.Astuti S. D. et al. Antimicrobial photodynamic effects of polychromatic light activated by magnetic fields to bacterial viability, Journal of International Dental and Medical Research, 2017, vol. 10(1), pp. 111-117.Rao L. et al. Investigating the inactivation mechanism of Bacillus subtilis spores by high pressure CO2, Frontiers in Microbiology, 2016, vol. 7, p. 1411.Rao L. et al. Investigating the inactivation mechanism of Bacillus subtilis spores by high pressure CO2, Frontiers in Microbiology, 2016, vol. 7, p. 1411.Adji D. and Larashanty H. Comparison of the effectiveness of 70% alcohol, infrared, autoclave and ozone sterilization on the growth of Bacillus subtilis bacteria, Journal of Veterinary Science, 2007, vol. 25(1).Adji D. and Larashanty H. Comparison of the effectiveness of 70% alcohol, infrared, autoclave and ozone sterilization on the growth of Bacillus subtilis bacteria, Journal of Veterinary Science, 2007, vol. 25(1).Astuti, S. D. et al. Photodynamic effectiveness of laser diode combined with ozone to reduce Staphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves, Biomedical Photonics, 2019, vol. 8(2), pp. 4-13.Astuti, S. D. et al. Photodynamic effectiveness of laser diode combined with ozone to reduce Staphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves, Biomedical Photonics, 2019, vol. 8(2), pp. 4-13.Ernie M.S. et al. An in vitro Anti-microbial Photodynamic Therapy (APDT) with Blue LEDs to activate chlorophylls of Alfalfa Medicago Sativa L on Aggregatibacter actinomycetemcomitans, Journal of International Dental and Medical Research, 2016, vol. 9(2), pp. 118-125.Ernie M.S. et al. An in vitro Anti-microbial Photodynamic Therapy (APDT) with Blue LEDs to activate chlorophylls of Alfalfa Medicago Sativa L on Aggregatibacter actinomycetemcomitans, Journal of International Dental and Medical Research, 2016, vol. 9(2), pp. 118-125.Hoenes K. et al. Microbial photoinactivation by visible light results in limited loss of membrane integrity, Antibiotics, 2021, vol. 10(3), p. 341.Hoenes K. et al. Microbial photoinactivation by visible light results in limited loss of membrane integrity, Antibiotics, 2021, vol. 10(3), p. 341.Astuti S. D. at al. Effectiveness Photodynamic Inactivation with Wide Spectrum Range of Diode Laser to Staphylococcus aureus Bacteria with Endogenous Photosensitizer: An in vitro Study, Journal of International Dental and Medical Research, 2019, vol. 12(2), pp. 481-486.Astuti S. D. at al. Effectiveness Photodynamic Inactivation with Wide Spectrum Range of Diode Laser to Staphylococcus aureus Bacteria with Endogenous Photosensitizer: An in vitro Study, Journal of International Dental and Medical Research, 2019, vol. 12(2), pp. 481-486.Habermeyer B. et al. Bactericidal effciency of porphyrin systems, Journal of Porphyrins and Phthalocyanines, 2021, vol. 25(05n06), pp. 359-381.Habermeyer B. et al. Bactericidal effciency of porphyrin systems, Journal of Porphyrins and Phthalocyanines, 2021, vol. 25(05n06), pp. 359-381.Caires C. S. et al. Photoinactivation effect of eosin methylene blue and chlorophyllin sodium-copper against Staphylococcus aureus and Escherichia coli, Lasers in medical science, 2017, vol. 32(5), pp. 1081-1088.Caires C. S. et al. Photoinactivation effect of eosin methylene blue and chlorophyllin sodium-copper against Staphylococcus aureus and Escherichia coli, Lasers in medical science, 2017, vol. 32(5), pp. 1081-1088.Astuti, S. D. et al. The effectiveness of nano-doxycycline Activated by Diode Laser Exposure to Reduce S. aureus Biofilms: an in vitro Study, In Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 2019, vol. 10863, pp. 180-191.Astuti, S. D. et al. The effectiveness of nano-doxycycline Activated by Diode Laser Exposure to Reduce S. aureus Biofilms: an in vitro Study, In Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 2019, vol. 10863, pp. 180-191.Sunarko S. A. et al. antimicrobial effect of pleomeleangustifolia pheophytin A activation with diode laser to streptococcus mutans, In Journal of Physics: Conference Series, 2017, vol. 853 (1), p. 012039. IOP Publishing.Sunarko S. A. et al. antimicrobial effect of pleomeleangustifolia pheophytin A activation with diode laser to streptococcus mutans, In Journal of Physics: Conference Series, 2017, vol. 853 (1), p. 012039. IOP Publishing.Rozykulyyeva L. et al. Antibacterial activities of green synthesized silver nanoparticles from Punica granatum peel extract, In AIP Conference Proceedings, 2020, vol. 2314 (1), p. 060012. AIP Publishing LLC.Rozykulyyeva L. et al. Antibacterial activities of green synthesized silver nanoparticles from Punica granatum peel extract, In AIP Conference Proceedings, 2020, vol. 2314 (1), p. 060012. AIP Publishing LLC.Dai Q. et al. Carrier recombination mechanisms and efficiency droop in GaInN/GaN light-emitting diodes, Applied Physics Letters, 2010, vol. 97(13), p. 133507.Dai Q. et al. Carrier recombination mechanisms and efficiency droop in GaInN/GaN light-emitting diodes, Applied Physics Letters, 2010, vol. 97(13), p. 133507.Mardianto A. I. et al. Photodynamic Inactivation of Streptococcus mutan Bacteri with Photosensitizer Moringa oleifera Activated by Light Emitting Diode (LED), In Journal of Physics: Conference Series, 2020, vol. 1505(1), p. 012061. IOP Publishing.Mardianto A. I. et al. Photodynamic Inactivation of Streptococcus mutan Bacteri with Photosensitizer Moringa oleifera Activated by Light Emitting Diode (LED), In Journal of Physics: Conference Series, 2020, vol. 1505(1), p. 012061. IOP Publishing.Semyonov D.Yu., Vasil’ev Yu.L., Dydykin S.S., Stranadko E.F., Shubin V.K., Bogomazov Yu.K., Morokhotov V.A., Shcherbyuk A.N., Morozov S.V., Zakharov Yu.I. Antimicrobial and antimycotic photodynamic therapy (review of literature), Biomedical Photonics, 2021, vol. 10(1), pp. 25-31. doi: 10.24931/2413-9432-2021-10-1-25-31Semyonov D.Yu., Vasil’ev Yu.L., Dydykin S.S., Stranadko E.F., Shubin V.K., Bogomazov Yu.K., Morokhotov V.A., Shcherbyuk A.N., Morozov S.V., Zakharov Yu.I. Antimicrobial and antimycotic photodynamic therapy (review of literature), Biomedical Photonics, 2021, vol. 10(1), pp. 25-31. doi: 10.24931/2413-9432-2021-10-1-25-31Maclean M. et al. Sporicidal effects of high-intensity 405 nm visible light on endospore-forming bacteria, Photochemistry and photobiology, 2013, vol. 89(1), pp. 120-126.Maclean M. et al. Sporicidal effects of high-intensity 405 nm visible light on endospore-forming bacteria, Photochemistry and photobiology, 2013, vol. 89(1), pp. 120-126.Maclean M. et al. 405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control, Journal of Hospital Infection, 2014, vol. 88(1), pp. 1-11.Maclean M. et al. 405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control, Journal of Hospital Infection, 2014, vol. 88(1), pp. 1-11.Endarko E. et al. High-intensity 405 nm light inactivation of Listeria monocytogenes, Photochemistry and photobiology, 2012, vol. 88(5), pp. 1280-1286.Endarko E. et al. High-intensity 405 nm light inactivation of Listeria monocytogenes, Photochemistry and photobiology, 2012, vol. 88(5), pp. 1280-1286.Plaetzer, K. et al. Photophysics and photochemistry of photodynamic therapy: fundamental aspects, Lasers in medical science, 2009, vol. 24(2), pp. 259-268.Plaetzer, K. et al. Photophysics and photochemistry of photodynamic therapy: fundamental aspects, Lasers in medical science, 2009, vol. 24(2), pp. 259-268.Kwiatkowski S. et al. Photodynamic therapy–mechanisms, photosensitizers and combinations, Biomedicine & pharmacotherapy, 2018, vol. 106, pp. 1098-1107.Kwiatkowski S. et al. Photodynamic therapy–mechanisms, photosensitizers and combinations, Biomedicine & pharmacotherapy, 2018, vol. 106, pp. 1098-1107.Juzeniene A. and Moan J. The history of PDT in Norway: Part one: Identification of basic mechanisms of general PDT, Photodiagnosis and photodynamic therapy, 2007, vol. 4(1), pp. 3-11.Juzeniene A. and Moan J. The history of PDT in Norway: Part one: Identification of basic mechanisms of general PDT, Photodiagnosis and photodynamic therapy, 2007, vol. 4(1), pp. 3-11.Wainwright M. et al. Photoantimicrobials are we afraid of the light? The Lancet Infectious Diseases, 2017, vol. 17(2), pp. e49-e55.Wainwright M. et al. Photoantimicrobials are we afraid of the light? The Lancet Infectious Diseases, 2017, vol. 17(2), pp. e49-e55.Mamone L et al. Photodynamic inactivation of Gram-positive bacteria employing natural resources, Journal of Photochemistry and Photobiology B: Biology, 2014, vol. 133, pp. 80-89.Mamone L et al. Photodynamic inactivation of Gram-positive bacteria employing natural resources, Journal of Photochemistry and Photobiology B: Biology, 2014, vol. 133, pp. 80-89.Sperandio F. et al. Antimicrobial photodynamic therapy to kill Gramnegative bacteria, Recent patents on anti-infective drug discovery, 2013, vol. 8(2), pp. 108-120.Sperandio F. et al. Antimicrobial photodynamic therapy to kill Gramnegative bacteria, Recent patents on anti-infective drug discovery, 2013, vol. 8(2), pp. 108-120.

The authors declare that there are no conflicts of interest present.