biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2022-11-4-11-18bioph-563Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESРазработка метода оценки глубины проникновения этосом с метиленовым синим в кожу при аппликационном применении и фотодинамическим воздействииDevelopment of a method for assessing the depth of penetration of ethosomes with methylene blue into the skin during application and photodynamic exposureЛогиноваА. Г.LoginovaA. G.

Москва

Moscow

НикитенкоИ. С.NikitenkoI. S.

Москва

Moscow

ТихоновскийГ. В.TikhonovskyG. V.

Москва

Moscow

СкобельцинА. С.SkobeltsinA. S.

Москва

Moscow

ВойтоваА. В.VoitovaA. V.

Москва

Moscow

ЛощеновВ. Б.LoschenovV. B.

Москва

Moscow

loschenov@mail.ruНациональный исследовательский ядерный университет «МИФИ»РоссияNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute)Russian FederationКлиника эстетической медицины «ЭстеМед»РоссияClinic of Aesthetic Cosmetology and Dermatovenerology LLC «EsteMed»Russian FederationНациональный исследовательский ядерный университет «МИФИ»; Институт общей физики им. А. М. Прохорова Российской академии наукРоссияNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Prokhorov General Physics Institute of Russian Academy of SciencesRussian FederationООО «БИОСПЕК»РоссияLLC BIOSPECRussian Federation2022310120231141118Copyright © Логинова А.Г., Никитенко И.С., Тихоновский Г.В., Скобельцин А.С., Войтова А.В., Лощенов В.Б., 20232023Логинова А.Г., Никитенко И.С., Тихоновский Г.В., Скобельцин А.С., Войтова А.В., Лощенов В.Б.Loginova A.G., Nikitenko I.S., Tikhonovsky G.V., Skobeltsin A.S., Voitova A.V., Loschenov V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/563

В широком спектре литературных источников сообщается о потенциальных преимуществах трансдермальной доставки лекарственных веществ. Среди данных преимуществ выделяют следующие – минимальная травматичность, снижение побочных эффектов, предотвращение деградации или метаболизма в желудочно-кишечном тракте или печени. Однако трансдермальная доставка большинства молекул часто исключается из-за барьерной функции кожи, которая препятствует проникновению экзогенных веществ. Для преодоления данного барьера и увеличения кожного поглощения могут быть использованы этосомальные комплексы, с помощью которых возможно проникновение в глубокие слои кожи и/или системное кровообращение. Данная работа посвящена разработке неинвазивного метода оценки глубины проникновения этосом с метиленовым синим в кожу при аппликационном применении и фотодинамическом воздействии. Именно метиленовый синий был выбран в качестве фотосенсибилизатора (ФС) в работе, поскольку имеется достаточное количество публикаций о его положительном влиянии на восстановление дыхательной цепи клеток различных органов и, тем самым восстановлении их метаболизма. Кроме того, метиленовый синий проявил себя как эффективный ФС, разрушающий патогенные микробы и вирусы, в том числе вирус SARS-CoV-2. Однако для более эффективной терапии Covid-19 и антибиотикорезистентных микробных заболеваний требуется проникновение метиленовый синий в сосудистую систему эпидермиса или слизистой ткани. На данный момент существующие методы оценки глубины проникновения фотосенсибилизаторов являются трудоёмкими и требуют использования кожи животных или модельных образцов. В работе была использована система ЛЭСА-01 БИОСПЕК со специально разработанными оптическими адапторами, позволяющими оценивать интенсивность флуоресценции препарата на поверхности кожи и на глубине до 2 мм.

A wide range of literature sources report on the potential benefits of transdermal drug delivery. Among these advantages, the following are distinguished – minimal injury, reduction of side effects, and prevention of degradation or metabolism in the gastrointestinal tract or liver. However, transdermal delivery of most molecules often excludes due to the barrier function of the skin, which prevents the penetration of exogenous substances. To overcome this barrier and increase skin absorption, ethosomal complexes use, by means penetration into the deep layers of the skin and/or systemic circulation is possible. This work devotes to the development of a non-invasive method for assessing the depth of penetration by ethosomes with methylene blue (MB) into the skin during application and photodynamic exposure. MB as photosensitizer (PS) was chosen, since there are a sufficient number of publications on its positive effect on the restoration of the cell’s respiratory chain of various organs and therefore the restoration of their metabolism. Besides MB has proven to be an effective PS, destructed pathogenic microbes and viruses, including SARS-CoV-2. However, for more effective Covid-19 therapy and antibiotic-resistant microbial diseases, the penetration of MB into the vascular system of the epidermis or mucous tissue is required. Nowadays, the existing methods for assessing the penetration depth of PS are time consuming and require the use of animal skin or model samples. The LESA-01 BIOSPEC system with specially designed optical adapters that allow assessing the drug fluorescence intensity on skin surface and at a depth of up to 2 mm in the investigation was used.

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The authors declare that there are no conflicts of interest present.