biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2023-12-1-4-13bioph-578Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСпектроскопическое исследование метиленового синего in vivo: влияние на оксигенацию тканей и опухолевый метаболизмSpectroscopic study of methylene blue in vivo: effects on tissue oxygenation and tumor metabolismПоминоваД. В.PominovaD. V.

Москва

Moscow

pominovadv@gmail.comРябоваА. В.RyabovaA. V.

Москва

Moscow

СкобельцинА. С.SkobeltsinA. S.

Москва

Moscow

МарковаИ. В.MarkovaI. V.

Москва

Moscow

РоманишкинИ. Д.RomanishkinI. D.

Москва

Moscow

ЛощеновВ. Б.LoschenovV. B.

Москва

Moscow

Институт общей физики им. А. М. Прохорова Российской академии наук; Национальный исследовательский ядерный университет «МИФИ»РоссияProkhorov General Physics Institute of Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)Russian FederationИнститут общей физики им. А. М. Прохорова Российской академии наукРоссияProkhorov General Physics Institute of Russian Academy of SciencesRussian FederationНациональный исследовательский ядерный университет «МИФИ»РоссияNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute)Russian Federation202309052023121413Copyright © Поминова Д.В., Рябова А.В., Скобельцин А.С., Маркова И.В., Романишкин И.Д., Лощенов В.Б., 20232023Поминова Д.В., Рябова А.В., Скобельцин А.С., Маркова И.В., Романишкин И.Д., Лощенов В.Б.Pominova D.V., Ryabova A.V., Skobeltsin A.S., Markova I.V., Romanishkin I.D., Loschenov V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/578

Метиленовый синий (МС) является перспективным фотосенсибилизатором для терапии патологических новообразований, поскольку обладает как фотодинамической активностью (при лазерном облучении), так и окислительно-восстановительными и каталитическими свойствами (в отсутствии света). В рамках данной работы при помощи спектроскопических методов было проанализировано влияние внутривенного введения МС на тканевую оксигенацию гемоглобина на малых животных in vivo в опухоли и нормальных тканях. Проведен анализ влияния МС на клеточный метаболизм. Показано, что применение МС способствует увеличению потребления кислорода опухолью, а также приводит к сдвигу метаболизма в сторону окислительного фосфорилирования.

Methylene blue (MB) is a promising photosensitizer (PS) for the treatment of pathological neoplasms, since it has both photodynamic activity (under laser irradiation) and redox and catalytic properties (in the absence of light). In the framework of this work, using spectroscopic methods, the effect of intravenous administration of MB on tissue oxygenation of hemoglobin in small animals in vivo in tumor and normal tissues was analyzed. The influence of MB on cell metabolism was analyzed. It has been shown that the use of MB promotes an increase in oxygen consumption by the tumor, and also leads to a shift in metabolism towards oxidative phosphorylation. It was shown that the use of MB contributes to an increase in oxygen consumption by the tumor, and also leads to a shift in metabolism towards oxidative phosphorylation.

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The authors declare that there are no conflicts of interest present.