biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2024-13-3-14-19bioph-658Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESНовый катионный хлорин как потенциальный агент для антимикробной фотодинамической терапииNew cationic chlorin as potential agent for antimicrobial photodynamic therapyСуворовН. В.SuvorovN. V.

Москва

Moscow

suvorov.nv@gmail.comЩелковаВ. В.ShchelkovaV. V.

Москва

Moscow

РусановаЕ. В.RysanovaE. V.

Москва

Moscow

БагателияЗ. Т.BagateliaZ. T.

Москва

Moscow

ДьяченкоД. А.DiachenkoD. A.

Москва

Moscow

АфанютинА. П.AfaniutinA. P.

Орел

Orel

ВасильевЮ. Л.Vasil’evYu. L.

Москва

Moscow

ДьячковаЕ. Ю.DiachkovaE. Yu.

Москва

Moscow

Сантана СантосИ. К.Santana SantosI. C.

Сан-Паулу

Sao Paulo

ГринМ. А.GrinM. A.

Москва

Moscow

Институт тонких химических технологий, РТУ МИРЭАРоссияInstitute of Fine Chemical Technologies, MIREA-Russian Technological UniversityRussian FederationМосковский областной научно-исследовательский клинический институт им. М.Ф. Владимирского; Российский государственный университет им. А.Н. КосыгинаРоссияMoscow Regional Research and Clinical Institute; N. Kosygin Russian State UniversityRussian FederationМосковский областной научно-исследовательский клинический институт им. М.Ф. ВладимирскогоРоссияMoscow Regional Research and Clinical InstituteRussian FederationПервый МГМУ им. И.М. Сеченова (Сеченовский Университет)РоссияI.M. Sechenov First Moscow State Medical University (Sechenov University)Russian FederationСтоматологическая клиника АвеДентРоссияAveDent, Dental ClinicRussian FederationИнститут тонких химических технологий, РТУ МИРЭА; Первый МГМУ им. И.М. Сеченова (Сеченовский Университет)РоссияInstitute of Fine Chemical Technologies, MIREA-Russian Technological University; I.M. Sechenov First Moscow State Medical University (Sechenov University)Russian FederationИнженерная школа Сан-Карлоса, Университет Сан-ПаулуБразилияSao Carlos School of Engineering, University of Sao PauloBrazil2024011120241331419Copyright © Суворов Н.В., Щелкова В.В., Русанова Е.В., Багателия З.Т., Дьяченко Д.А., Афанютин А.П., Васильев Ю.Л., Дьячкова Е.Ю., Сантана Сантос И.К., Грин М.А., 20242024Суворов Н.В., Щелкова В.В., Русанова Е.В., Багателия З.Т., Дьяченко Д.А., Афанютин А.П., Васильев Ю.Л., Дьячкова Е.Ю., Сантана Сантос И.К., Грин М.А.Suvorov N.V., Shchelkova V.V., Rysanova E.V., Bagatelia Z.T., Diachenko D.A., Afaniutin A.P., Vasil’ev Y.L., Diachkova E.Y., Santana Santos I.C., Grin M.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/658

Множественная устойчивость микроорганизмов к антибиотикам является одним из основных рисков для безопасности в области глобального здравоохранения. Нарастание резистентности бактерий к уже имеющимся препаратам поставили на повестку дня поиск альтернативных способов борьбы с антибиотикорезистентными возбудителями инфекций. Одним из таких инновационных методов является антимикробная фотодинамическая терапия (АФДТ) одинаково эффективная против антибиотикочувствительных и антибиотикорезистентных возбудителей. Наиболее эффективными фотосенсибилизаторами (ФС) для АФДТ являются молекулы, содержащие положительно-заряженные группы в своем составе. В настоящей работе нами было получено новое катионное производное природного хлорина, содержащее пиридазиновую группу в своем составе, введение которой происходит с использованием подходов clickхимии. Противомикробную фотоиндуцированную цитотоксичность предлагаемого катионного ФС, а также его незаряженного предшественника, оценивали в отношении ряда грамположительных и грамотрицательных бактерий: S. aureus, K. pneumoniae, E. faecalis, P. aeruginosa. Показано, что катионный хлорин обладает повышенным бактерицидным действием при облучении светом (λ =660 нм, Ps = 70,73 мВ/см2 ) по сравнению со своей основной формой. При инкубировании микробных суспензий с раствором катионного ФС в концентрации 24 мкМ и последующим облучением наблюдался заметный бактерицидный эффект в отношении всех вышеназванных бактерий. В результате проведенных микробиологических исследований показано, что предлагаемый катионный ФС обладает высокой фотоиндуцированной антимикробной активностью.

Multiple drug resistance is a major global health security risk. Increasing resistance of bacteria to existing drugs puts on the agenda the search for alternative ways to combat antibiotic-resistant pathogens. One of these innovative methods is antimicrobial photodynamic therapy (APDT), which is equally effective against antibiotic-sensitive and antibiotic-resistant pathogens. The most effective photosensitizers (PS) for APDT are molecules containing positively charged groups in their composition. In this work, we have obtained a new cationic derivative of natural chlorin containing a pyridazine group in its composition, the introduction of which occurs using click chemistry approaches. The antimicrobial photoinduced cytotoxicity of the proposed cationic PS, as well as its uncharged precursor, was assessed against a number of gram-positive and gram-negative bacteria: S. aureus, K. pneumoniae, E. faecalis, P. aeruginosa. It has been shown that cationic chlorin exhibits an increased bactericidal effect when irradiated with light (λ = 660 nm, Ps = 70.73 mW/cm2) compared to its base form. When microbial suspensions were incubated with 24 μM cationic PS and subsequently irradiated, a significant bactericidal effect was observed against all of the aforementioned bacteria. As a result of microbiological studies, it was demonstrated that the proposed cationic PS exhibits high photoinduced antimicrobial activity.

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The authors declare that there are no conflicts of interest present.