biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2024-13-4-4-12bioph-676Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСравнение оптико-спектральных характеристик глиобластомы при интраоперационной диагностике и оптической биопсии ex vivoComparison of optical-spectral characteristics of glioblastoma at intraoperative diagnosis and ex vivo optical biopsyРоманишкинИ. Д.RomanishkinI. D.

Москва

Moscow

igor.romanishkin@nsc.gpi.ruСавельеваТ. А.SavelievaT. A.

Москва

Moscow

ОспановА.OspanovA.

Москва

Moscow

КалягинаН. А.KalyaginaN. A.

Москва

Moscow

КривецкаяА. А.KrivetskayaA. A.

Москва

Moscow

УденеевА. М.UdeneevA. M.

Москва

Moscow

ЛиньковК. Г.LinkovK. G.

Москва

Moscow

ГоряйновС. А.GoryajnovS. A.

Москва

Moscow

ШугайС. В.ShugayS. V.

Москва

Moscow

ПавловаГ. В.PavlovaG. V.

Москва

Moscow

ПронинИ. Н.ProninI. N.

Москва

Moscow

ЛощеновВ. Б.LoschenovV. B.

Москва

Moscow

Институт общей физики им. А.М. Прохорова Российской академии наукРоссияProkhorov General Physics Institute of Russian Academy of SciencesRussian FederationИнститут общей физики им. А.М. Прохорова Российской академии наук; Национальный исследовательский ядерный университет «МИФИ»РоссияProkhorov General Physics Institute of Russian Academy of Sciences; National Research Nuclear University MEPhIRussian FederationНациональный исследовательский ядерный университет «МИФИ»РоссияNational Research Nuclear University MEPhIRussian FederationНациональный медицинский исследовательский центр нейрохирургии имени академика Н. Н. БурденкоРоссияN.N. Burdenko National Medical Research Center of NeurosurgeryRussian FederationНациональный медицинский исследовательский центр нейрохирургии имени академика Н. Н. Бурденко; Институт высшей нервной деятельности и нейрофизиологии Российской академии наукРоссияN.N. Burdenko National Medical Research Center of Neurosurgery; Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of SciencesRussian Federation202425122024134412Copyright © Романишкин И.Д., Савельева Т.А., Оспанов А., Калягина Н.А., Кривецкая А.А., Уденеев А.М., Линьков К.Г., Горяйнов С.А., Шугай С.В., Павлова Г.В., Пронин И.Н., Лощенов В.Б., 20242024Романишкин И.Д., Савельева Т.А., Оспанов А., Калягина Н.А., Кривецкая А.А., Уденеев А.М., Линьков К.Г., Горяйнов С.А., Шугай С.В., Павлова Г.В., Пронин И.Н., Лощенов В.Б.Romanishkin I.D., Savelieva T.A., Ospanov A., Kalyagina N.A., Krivetskaya A.A., Udeneev A.M., Linkov K.G., Goryajnov S.A., Shugay S.V., Pavlova G.V., Pronin I.N., Loschenov V.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/676

Сложность интраоперационного определения границ глиальных опухолей обусловлена особенностями их роста вдоль сосудов и нервных волокон с инфильтрацией здорового белого вещества. При этом недостаточно полное удаление опухолевых тканей приводит к рецидивам, а избыточное удаление чревато неврологическими осложнениями. Методы оптической спектроскопии характеризуются высокой скоростью, точностью и неинвазивностью, что обуславливает перспективность их использования для интраоперационной демаркации границ таких опухолей. Спектроскопия флуоресценции и диффузного отражения нашли широкое применение в интраоперационной нейронавигации, главным образом, для обнаружения краев диффузных глиом. При этом в последние годы активно развивается направление ex vivo спектрального анализа образцов опухолей с помощью сочетания различных методов оптической спектроскопии, включающих спектроскопию как упругого, так и неупругого рассеяния. Очевидно, возможности регистрации спектров при интраоперационной работе и на свежих образцах отличаются. В настоящей статье проведено сравнение результатов анализа оптико-спектральных характеристик внутричерепных опухолей при интраоперационной диагностике и анализе ex vivo, а также предложена математическая модель для интерпретации наблюдаемых зависимостей.

The difficulty of intraoperative delineation of glial tumors is due to the peculiarities of their growth along vessels and nerve fibers with infiltration of healthy white matter. Insufficiently complete removal of tumor tissues leads to recurrences, and excessive removal is fraught with neurological complications. Optical spectroscopy methods are characterized by high speed, accuracy and non-invasiveness, which determines the prospects of their use for intraoperative demarcation of the boundaries of such tumors. Fluorescence and diffuse reflectance spectroscopy have found wide application in intraoperative neuronavigation, mainly for detecting the edges of diffuse gliomas. At the same time, in recent years the direction of <i>ex vivo</i> spectral analysis of tumor samples using a combination of various optical spectroscopy methods, including both elastic and inelastic scattering spectroscopy, has been actively developed. Obviously, the ability to obtain spectra intraoperatively and on fresh specimens is different. The present article compares the results of the analysis of optical-spectral characteristics of intracranial tumors at intraoperative diagnosis and <i>ex vivo</i> analysis and proposes a mathematical model for interpretation of the observed dependencies.

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The authors declare that there are no conflicts of interest present.