biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2025-14-2-12-20bioph-716Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЛокальная анизотропия рассеяния кожи как возможный фактор искажения флуоресцентных границ опухолиLocal scattering anisotropy of the skin as a possible factor of fluorescence borders distortion of neoplasmsКирющенковаН. П.KiryushchenkovaN. P.

Москва

Moscow

nkir@niigb.ruНовиковИ. А.NovikovI. A.

Москва

Moscow

Научно-исследовательский институт глазных болезней им. М.М. КрасноваРоссияM.M. Krasnov Research Institute of Eye DiseasesRussian FederationИнститут общей физики им. А.М. Прохорова Российской академии наукРоссияM.M. Krasnov Research Institute of Eye Diseases; Prokhorov General Physics Institute of Russian Academy of SciencesRussian Federation2025010720251421220Copyright © Кирющенкова Н.П., Новиков И.А., 20252025Кирющенкова Н.П., Новиков И.А.Kiryushchenkova N.P., Novikov I.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/716

Широкое применение диагностики новоо6разований кожи на основе анализа флуоресценции протопорфирина IX ограничено сложностью распространения, в частности рассеяния, света в тканях. Для оценки влияния локальной анизотропии рассеяния кожи на картину флуоресценции опухоли предлагается производить сравнение последней с флуоресцентной картиной распространения света от точечного источника, приложенного к поверхности кожи в проекции опухоли. Такой тест 6ыл 6ы полезен для выявления случаев скрытого роста новоо6разования, однако, репрезентативность его неизвестна. B описанном здесь эксперименте изучалась корреляция между паттернами рассеяния света от внешнего и внутреннего источника в пределах одного и того же участка кожи. Моделью служила голова свиньи. Четыре зоны интереса с различными оптическими свойствами 6ыли подо6раны с учетом строения средней трети лица чело- века. Длина волны источника света 6ыла вы6рана так, что6ы имитировать флуоресценцию протопорфирина IX. Соответствие моделей распределения света 6ыло определено количественно корреляционным методом. Полученные результаты наглядно продемонстрировали сильную взаимосвязь между характером распределения флуоресценции опухоли и состоянием/топографией окружающих тканей и доказали возможность использования внешнего источника света для оценки локальной анизотропии рассеяния кожи in vivo.

The use of protoporphyrin IX fluorescence imaging in skin tumors is limited by the complexity of light propagation in tissues. A non-invasive scat- tering anisotropy test (comparison of the fluorescence pattern of a tumor with that of a point source applied to the same spot) would be useful in distinguishing between cases of subsurface tumor growth and local fluorescence pattern distortions. However, the knowledge is missing of whether the distribution from an external light source would be representative. The experiment described here addressed the correlation between patterns in which light is dispersed from an external and an internal source within the same area of the skin. A pig’s head was chosen as the model. Four zones of interest were identified, all different in optical properties. The wavelength of the light source was selected as to simulate the PpIX fluorescence. The correspondence of light distribution patterns was quantified using the correlation method. The results have clearly demon- strated the strong relationship between the fluorescence distribution pattern of a tumor and the condition/topography of the surrounding tissues and proved the possibility of using an external light source to assess the local scattering anisotropy of the skin in vivo.

анизотропия рассеянияновоо6разование кожифлуоресценцияграница опухолипротопорфирин IXscattering anisotropyskin neoplasmfluorescencetumor borderprotoporphyrin IXReferencesKaprin A.D., Starinsky V.V., Shakhzadova A.O. eds. Malignant neoplasms in Russia in 2020 (morbidity and mortality). M.: P.A. Herzen Moscow Oncology Research Institute. – 2021. (In Russ.)Kaprin A.D., Starinsky V.V., Shakhzadova A.O. eds. Malignant neoplasms in Russia in 2020 (morbidity and mortality). M.: P.A. Herzen Moscow On- cology Research Institute. – 2021. (In Russ.)Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries // CA Cancer J Clin. – 2021. – Vol.71. – P. 209-249. https://doi.org/10.3322/caac.21660Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 2021, Vol. 71, рр. 209-249. https://doi.org/10.3322/caac.21660Peris K., Fargnoli M.C., Garbe C., Kaufmann R., Bastholt L., Seguin N.B., Bataille V., Marmol V. D., Dummer R., Harwood C.A., Hauschild A., Höller C., Haedersdal M., Malvehy J., Middleton M.R., Morton C.A., Nagore E., Stratigos A.J., Szeimies R.M., Tagliaferri L., Trakatelli M., Zalaudek I., Eg- germont A., Grob J.J. Diagnosis and treatment of basal cell carcinoma: European consensus-based interdisciplinary guidelines // Eur J Cancer. – 2019. – Vol. 118. – P.10-34.Peris K., Fargnoli M.C., Garbe C., Kaufmann R., Bastholt L., Seguin N.B., Bataille V., Marmol V. D., Dummer R., Harwood C.A., Hauschild A., Höller C., Haedersdal M., Malvehy J., Middleton M.R., Morton C.A., Nagore E., Stratigos A.J., Szeimies R.M., Tagliaferri L., Trakatelli M., Zalaudek I., Eg- germont A., Grob J.J. Diagnosis and treatment of basal cell carcinoma: European consensus-based interdisciplinary guidelines. Eur J Cancer, 2019, Vol. 118, рр. 10-34.Vornicescu C., Șenilă S.C., Bejinariu N.I., VesaȘ.C., Boșca A.B., Chirilă D.N., MelincoviciC.S., Sorițău O., Mihu C.M. Predictive factors for the recur- rence of surgically excised basal cell carcinomas: A retrospective clini- cal and immunopathological pilot study // Experimental and Thera- peutic Medicine. – 2021. – Vol. 22(5). – P.1336. https://doi.org/10.3892/ etm.2021.10771Vornicescu C., Șenilă S.C., Bejinariu N.I., Vesa Ș.C., Boșca A.B., Chirilă D.N., MelincoviciC.S., Sorițău O., Mihu C.M. Predictive factors for the recurrence of surgically excised basal cell carcinomas: A retrospective clinical and immunopathological pilot study. Experimental and Thera- peutic Medicine, 2021, Vol. 22(5), рр.1336. https://doi.org/10.3892/ etm.2021.10771Policard A. Etudes sur les aspects offerts par des tumeurs experimen- tales ex aminées a la lumière de Wood // C R Soc Biol. – 1924. – Vol. 91. – P. 1423-1428.Policard A. Etudes sur les aspects offerts par des tumeurs experimen- tales ex aminées a la lumière de Wood. C R Soc Biol, 1924, Vol. 91, рр. 1423-1428.GalkinaE.M., Utz S.R. Fluorescence diagnosis in dermatology (review) // Saratov Journal of Medical Scientific Research. – 2013. – Vol. 9(3). – P. 566-572. (In Russ.)GalkinaE.M., Utz S.R. Fluorescence diagnosis in dermatology (review). Saratov Journal of Medical Scientific Research, 2013, Vol. 9(3), рр. 566-572. (In Russ.)Wizenty J., Schumann T., Theil, D., Stockmann M., Pratschke J., Tacke F., Aigner F., Wuensch T. Recent Advances and the Potential for Clini- cal Use of Autofluorescence Detection of Extra-Ophthalmic Tissues // Molecules. – 2020. – Vol. 25(9). – P. 2095.Wizenty J., Schumann T., Theil, D., Stockmann M., Pratschke J., Tacke F., Aigner F., Wuensch T. Recent Advances and the Potential for Clinical Use of Autofluorescence Detection of Extra-Ophthalmic Tissues. Molecules, 2020, Vol. 25(9), рр. 2095.Croce A.C., Bottiroli G. Autofluorescence spectroscopy and imaging: A tool for biomedical research and diagnosis // Eur. J. Histochem. – 2014. – Vol. 58. – P. 2461.Croce A.C., Bottiroli G. Autofluorescence spectroscopy and imaging: A tool for biomedical research and diagnosis. Eur. J. Histochem, 2014, Vol. 58. – P. 2461.Vo-Dinh T. Biomedical photonics handbook: biomedical diagnostics // CRC press. Boca Raton. – 2014.Vo-Dinh T. Biomedical photonics handbook: biomedical diagnostics. CRC press. Boca Raton. – 2014.McNicholas K., MacGregor M.N., Gleadle J.M. In order for the light to shine so brightly, the darkness must be present-why do cancers fluo- resce with 5-aminolaevulinic acid? // Br J Cancer. – 2019. – Vol. 121(8). – P. 631-639. https://doi.org/10.1038/s41416-019-0516-4.McNicholas K., MacGregor M.N., Gleadle J.M. In order for the light to shine so brightly, the darkness must be present-why do cancers fluo- resce with 5-aminolaevulinic acid? Br J Cancer, 2019, Vol. 121(8), рр. 631-639. https://doi.org/10.1038/s41416-019-0516-4.Pavlova N.N., Thompson C.B. The emerging hallmarks of cancer metabolism // Cell Metab. – 2016. – Vol. 23 (1). – P.27-47. https://doi. org/10.1016/j.cmet.2015.12.006.Pavlova NN, Thompson CB. The emerging hallmarks of cancer metabo- lism. Cell Metab, 2016, Vol. 23(1), рр. 27-47. https://doi.org/10.1016/j. cmet.2015.12.006.Potapov A.A., Goriaĭnov S.A., Loshchenov V.B., Savel'eva T.A., Gavrilov A.G., Okhlopkov V.A., Zhukov V.Iu., Zelenkov P.V., Gol'bin D.A., Shurkhaĭ V.A., Shishkina L.V., Grachev P.V., Kholodtsova M.N., Kuz'min S.G., Voro- zhtsov G.N., Chumakova A.P. Intraoperative combined spectroscopy (optical biopsy) of cerebral gliomas // ZhVoprNeirokhirIm N. N. Bur- denko. – 2013. – Vol. 77(2). – P. 3-10. (In Eng., Russ.)Potapov A.A., Goriaĭnov S.A., Loshchenov V.B., Savel'eva T.A., Gavrilov A.G., Okhlopkov V.A., Zhukov V.Iu., Zelenkov P.V., Gol'bin D.A., Shurkhaĭ V.A., Shishkina L.V., Grachev P.V., Kholodtsova M.N., Kuz'min S.G., Voro- zhtsov G.N., Chumakova A.P. Intraoperative combined spectroscopy (optical biopsy) of cerebral gliomas. ZhVoprNeirokhirIm N. N. Burdenko, 2013, Vol. 77(2), рр. 3-10. (In Eng., Russ.)Na R., Stender I.M., Wulf H.C. Can autofluorescence demarcate basal cell carcinoma from normal skin? A comparison with protoporphyrin IX fluorescence // Acta Derm Venereol. – 2001. – Vol. 81. – P. 246-249. https://doi.org/10.1080/00015550152572859Na R., Stender I.M., Wulf H.C. Can autofluorescence demarcate basal cell carcinoma from normal skin? A comparison with protoporphyrin IX fluorescence. Acta Derm Venereol, 2001, Vol. 81, рр. 246-249. https:// doi.org/10.1080/00015550152572859Hegyi J., Hegyi V., New developments in fluorescence diagnostics. In: Michael R. Hamblin, Pinar Avci, Gaurav K. Gupta, eds // Imaging in Der- matology. Academic Press. – 2016. – P. 89-94. https://doi.org/10.1016/ B978-0-12-802838-4.00009-1Hegyi J., Hegyi V., New developments in fluorescence diagnostics. In: Michael R. Hamblin, Pinar Avci, Gaurav K. Gupta, eds. Imaging in Derma- tology. Academic Press, 2016, рр. 89-94. https://doi.org/10.1016/B978- 0-12-802838-4.00009-1Grusha Ia.O., Kiryushchenkova N.P., Novikov I.A., Fedorov A.A., Ismailo- va D.S. Histological verification of autofluorescence borders of perior- bital skin tumors // Vestnik Oftalmologii. – 2020. – Vol. 136(6). – P. 32-41. (In Russ.) https://doi.org/10.17116/oftalma202013606132Grusha Ia.O., Kiryushchenkova N.P., Novikov I.A., Fedorov A.A., Ismailova D.S. Histological verification of autofluorescence borders of periorbital skin tumors. Vestnik Oftalmologii, 2020, Vol. 136(6), рр. 32-41. (In Russ.) https://doi.org/10.17116/oftalma202013606132Kienle A., Foschum F., Hohmann A. Light propagation in structural anisotropic media in the steady-state and time domains // Phys. Med. Biol. – 2013. – Vol. 58(17). – P. 6205. https://doi.org/10.1088/0031- 9155/58/17/6205Kienle A., Foschum F., Hohmann A. Light propagation in struc- tural anisotropic media in the steady-state and time domains. Phys. Med. Biol, 2013, Vol. 58(17), рр. 6205. https://doi.org/10.1088/0031- 9155/58/17/6205Jacques S.L. Optical properties of biological tissues: a review // Phys Med Biol. – 2013. – Vol. 58(11). – P. 37-61. https://doi.org/0.1088/0031- 9155/58/11/R37.Jacques S.L. Optical properties of biological tissues: a review. Phys Med Biol, 2013, Vol. 58(11), рр. 37-61. https://doi.org/0.1088/0031- 9155/58/11/R37.Nickell S. et al. Anisotropy of light propagation in human skin // Phys. Med. Biol. – 2000. – Vol. 45. – P. 2873-2886.Nickell S. et al. Anisotropy of light propagation in human skin. Phys. Med. Biol, 2000, Vol. 45, рр. 2873-2886.Tuchin V.V. Lasers and optical fibers in biomedical studies (2d ed.) // Fizmatlit. – 2010 (In Russ.)Tuchin V.V. Lasers and optical fibers in biomedical studies (2d ed.). Fiz- matlit, 2010 (In Russ.)Tuchin V.V. Optical biomedical diagnosis // Izvestiya of Saratov Uni- versity. Physics. – 2005. – Vol. 1(5). – P. 39-53. (In Russ.) https://doi. org/10.18500/1817-3020-2005-5-1-39-53.Tuchin V.V. Optical biomedical diagnosis. Izvestiya of Saratov Uni- versity. Physics, 2005, Vol. 1(5), рр. 39-53. (In Russ.) https://doi. org/10.18500/1817-3020-2005-5-1-39-53.Anderson RR, Parrish JA. The optics of human skin // J Invest Derma- tol. – 1981. – Vol.77 (1). – P. 13-9. https://doi.org/10.1111/1523-1747. ep12479191.Anderson RR, Parrish JA. The optics of human skin. J Invest Derma- tol, 1981, Vol. 77 (1), рр. 13-9. https://doi.org/10.1111/1523-1747. ep12479191.Colas V., Daul C., Khairallah G., Amouroux M., Blondel W. Spatially re- solved diffuse reflectance and autofluorescence photon depth dis- tribution in human skin spectroscopy: a modeling study // Proc. SPIE 11553.- Optics in Health Care and Biomedical Optics X. – 115531A. https://doi.org/10.1117/12.2575069Colas V., Daul C., Khairallah G., Amouroux M., Blondel W. Spatially re- solved diffuse reflectance and autofluorescence photon depth dis- tribution in human skin spectroscopy: a modeling study. Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115531A. https:// doi.org/10.1117/12.2575069Brancaleon L., Durkin A.J., Tu J.H., Menaker G., Fallon J.D., Kollias N. In vivo Fluorescence Spectroscopy of Nonmelanoma Skin Cancer // Pho- tochemistry and Photobiology. –2001. – Vol. 73(2). – P. 178-183. https:// doi.org/10.1562/0031-8655(2001)073<0178: IVFSON>2.0.CO;2Brancaleon L., Durkin A.J., Tu J.H., Menaker G., Fallon J.D., Kollias N. In vivo Fluorescence Spectroscopy of Nonmelanoma Skin Cancer. Photo- chemistry and Photobiology, 2001, Vol. 73(2), рр. 178-183. https://doi. org/10.1562/0031-8655(2001)073Masuda Y., Ogura Y., Inagaki Y., Yasui T., Aizu Y. Analysis of the influence of collagen fibres in the dermis on skin optical reflectance by Monte Carlo simulation in a nine-layered skin model // Skin Res Technol. – 2018. – Vol. 24. – P. 248-255. https://doi.org/10.1111/srt.12421Masuda Y., Ogura Y., Inagaki Y., Yasui T., Aizu Y. Analysis of the influence of collagen fibres in the dermis on skin optical reflectance by Monte Carlo simulation in a nine-layered skin model. Skin Res Technol, 2018, Vol. 24, рр. 248-255. https://doi.org/10.1111/srt.12421Smirnova O.D., Rogatkin D., Litvinova K. Collagen as in vivo quantita- tive fluorescent biomarkers of abnormal tissue changes // J. Innov. Opt. Health Sci. – 2012. – Vol. 05(2). – P.1250010.Smirnova O.D., Rogatkin D., Litvinova K. Collagen as in vivo quantita- tive fluorescent biomarkers of abnormal tissue changes. J. Innov. Opt. Health Sci, 2012, Vol. 05(2), рр.1250010.Yagi R., Kawabata S., Ikeda N. et al. Intraoperative 5-aminolevulinic acid- induced photodynamic diagnosis of metastatic brain tumors with his- topathological analysis // World J SurgOnc – 2017. – Vol. 15 – P. 179. https://doi.org/10.1186/s12957-017-1239-8Yagi R., Kawabata S., Ikeda N. et al. Intraoperative 5-aminolevulinic acid- induced photodynamic diagnosis of metastatic brain tumors with his- topathological analysis. World J SurgOnc, 2017, Vol. 15, рр.179. https:// doi.org/10.1186/s12957-017-1239-8Redondo P., Marquina M., Pretel M., Aguado L., Iglesias M.E. Methyl- ALA-Induced Fluorescence in Photodynamic Diagnosis of Basal Cell Carcinoma Prior to Mohs Micrographic Surgery // ArchDermatol. – 2008. – Vol. 144(1). – P. 115-117. https://doi.org/10.1001/archderma- tol.2007.3Redondo P., Marquina M., Pretel M., Aguado L., Iglesias M.E. Methyl- ALA-Induced Fluorescence in Photodynamic Diagnosis of Basal Cell Carcinoma Prior to Mohs Micrographic Surgery. ArchDermatol, 2008, Vol. 144(1), рр.115-117. https://doi.org/10.1001/archderma- tol.2007.3Kiryushchenkova N.P., Grusha Y., The use of autofluorescence diagnos- tics in monitoring and evaluating the effectiveness of local chemo- therapy for superficial basal cell carcinoma of the skin (clinical case) // Modern technologies in ophthalmology. – 2020. – Vol. 4 (35). – P. 162-163. (In Russ.) https://doi.org/10.25276/2312-4911-2020-4-162-163Kiryushchenkova N.P., Grusha Y., The use of autofluorescence diagnos- tics in monitoring and evaluating the effectiveness of local chemo- therapy for superficial basal cell carcinoma of the skin (clinical case). Modern technologies in ophthalmology, 2020, Vol. 4 (35), рр.162-163. (In Russ.) https://doi.org/10.25276/2312-4911-2020-4-162-163Hefti M., von Campe G., Moschopulosa M., Siegnerb A., Looserc H., Landolt H. 5-aminolaevulinic acid-induced protoporphyrin IX fluores- cence in high-grade glioma surgery // SWISS MED WKLY – 2008. –Vol. 138(11-12). – P. 180-185.Hefti M., von Campe G., Moschopulosa M., Siegnerb A., Looserc H., Landolt H. 5-aminolaevulinic acid-induced protoporphyrin IX fluo- rescence in high-grade glioma surgery. SWISS MED WKLY, 2008, Vol. 138(11-12), рр.180-185.Sapronov M.V., Skornyakova N.M. Computer visualization of Rayleigh scattering indicatrix in dynamic // Scientific visualization. – 2017. – Vol. 3(9). – P. 42-53. (In Russ.)Sapronov M.V., Skornyakova N.M. Computer visualization of Rayleigh scattering indicatrix in dynamic. Scientific visualization, 2017, Vol.3(9), рр.42-53. (In Russ.)Kalyagina N., Loschenov V., Wolf D., Daul C., Blondel W., Savelieva T. Ex- perimental and Monte Carlo investigation of visible diffuse-reflectance imaging sensitivity to diffusing particle size changes in an optical mod- el of a bladder wall // Applied Physics B. – 2011. – Vol. 105(3). – P. 631- 639.doi:10.1007/s00340-011-4678-xKalyagina N., Loschenov V., Wolf D., Daul C., Blondel W., Savelieva T. Ex- perimental and Monte Carlo investigation of visible diffuse-reflectance imaging sensitivity to diffusing particle size changes in an optical mod- el of a bladder wall. Applied Physics B, 2011, Vol. 105. (3), рр. 631-639. doi:10.1007/s00340-011-4678-xColas V., Amouroux M., Daul C., Perrin-Mozet C., Blondel W. Compara- tive study of optical properties estimation on liquid optical phantoms using spatially-resolved diffuse reflectance spectroscopy and double integrating spheres methods // Proc. SPIE 12147. – Tissue Optics and Photonics II. – P. 1214705. https://doi.org/10.1117/12.2621496Colas V., Amouroux M., Daul C., Perrin-Mozet C., Blondel W. Compara- tive study of optical properties estimation on liquid optical phantoms using spatially-resolved diffuse reflectance spectroscopy and double integrating spheres methods. Proc. SPIE 12147. – Tissue Optics and Pho- tonics II, рр. 1214705. https://doi.org/10.1117/12.2621496

The authors declare that there are no conflicts of interest present.