biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2025-14-2-21-26bioph-718Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESO зависимости интенсивности флуоресценции от концентрации растворов фотосенси6илизаторовOn the dependence of fluorescence intensity on the concentration of photosensitizer solutionsМееровичГ. А.MeerovichG. A.

Москва

Moscow

РоманишкинИ. Д.RomanishkinI. D.

Москва

Moscow

igor.romanishkin@nsc.gpi.ruАхлюстинаЕ. В.AkhlyustinaE. V.

Москва

Moscow

ЛощеновВ. Б.LoschenovV. B.

Москва

Moscow

Институт общей физики им. А. М. Прохорова Российской академии наукРоссияProkhorov General Physics Institute of Russian Academy of SciencesRussian FederationИнститут общей физики им. А. М. Прохорова Российской академии наук; Национальный исследовательский ядерный университет «МИФИ»РоссияProkhorov General Physics Institute of Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute),Russian FederationНациональный исследовательский ядерный университет «МИФИ»РоссияNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute)Russian FederationИнститут общей физики им. А. М. Прохорова Российской академии наук; Национальный исследовательский ядерный университет «МИФИ»РоссияProkhorov General Physics Institute of Russian Academy of Sciences;Russian Federation2025010720251422126Copyright © Меерович Г.А., Романишкин И.Д., Ахлюстина Е.В., Лощенов В.Б., 20252025Меерович Г.А., Романишкин И.Д., Ахлюстина Е.В., Лощенов В.Б.Meerovich G.A., Romanishkin I.D., Akhlyustina E.V., Loschenov V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/718

При исследовании оптических свойств фотосенси6илизаторов принято считать, что интенсивность их флуоресценции линейно зависит от концентрации. Oднако, есть много факторов, которые нео6ходимо учитывать. При низких концентрациях фотосенси6илиза- тора часть энергии воз6уждающего излучения, выходящая за пределы о6ъема воз6уждаемого раствора, теряется, а из-за локальной или «односторонней» регистрации часть изотропно распространяющегося излучения флуоресценции, также не регистрируется. При 6олее высоких концентрациях потери флуоресцентного света увеличиваются за счет перепоглощения его части молекулами фото- сенси6илизатора и последующего изотропного переизлучения с квантовым выходом значительно ниже 1, а дальнейшее увеличение концентрации приводит к частичной агрегации ФС, и к следующему из этого снижению эффективной флуоресценции. При высоком поглощении, флуоресценция воз6уждается только в ограниченном о6ъеме в6лизи источника воз6уждающего излучения, из-за чего 6ольшое значение начинают иметь геометрические осо6енности регистрации света. Это нео6ходимо учитывать при флуоресцентной диагностике и навигации с использованием данной характеристики.

When studying the optical properties of photosensitizers, it is assumed that their fluorescence intensity depends linearly on concentration. How- ever, there are many factors that need to be taken into account. At low photosensitizer concentrations, a part of the excitation radiation energy is lost beyond the volume of the excited solution, and due to local or one-directional registration, a large part of isotropically emitted fluorescence radiation is also not registered. At higher concentrations, the loss of fluorescence light increases due to its partial re-absorption by the photosensi- tizer molecules and subsequent isotropic re-emission with quantum yield much lower than 1, and further increase of concentration leads to partial aggregation of PS, and to the following decrease of effective fluorescence. At high absorption, fluorescence is excited only in a limited volume close to the excitation radiation source, leading to higher significance of light registration geometry. This should be taken into account in fluorescence diagnostics and navigation using this characteristic.

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The authors declare that there are no conflicts of interest present.