biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2025-14-3-24-29bioph-732Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESФотодинамическая эффективность in vitro поликатионных фотосенсибилизаторов на основе длинноволновых фталоцианиновIn vitro photodynamic efficacy of polycationic photosensitizers based on polycationic phthalocyaninesРоманишкинИ. Д.RomanishkinI. D.

Москва

Moscow 119991

igor.romanishkin@nsc.gpi.ruМееровичИ. Г.MeerovichI. G.

Москва

Moscow

БунинД. А.BuninD. A.

Москва

Moscow

СкобельцинА. С.SkobeltsinA. S.

Москва

Moscow 119991

АхлюстинаЕ. В.AkhlyustinaE. V.

Москва

Moscow

ЛевкинВ. В.LevkinV. V.

Москва

Moscow

ХарнасС. С.KharnasS. S.

Москва

Moscow

КоганЕ. А.KoganE. A.

Москва

Moscow

ChenZhi-LongChenZhi-Long

Шанхай

Shanghai

МееровичГ. А.MeerovichG. A.

Москва

Moscow 119991

ГорбуноваЮ. Г.GorbunovaYu. G.

Москва

Moscow

РешетовИ. В.ReshetovI. V.

Москва

Moscow

Институт общей физики им. А. М. Прохорова Российской академии наук; Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет)РоссияProkhorov General Physics Institute of the Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University (Sechenov University)Russian FederationИнститут биохимии им. А.Н. Баха Российской академии наукРоссияA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesRussian FederationПервый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет); Федеральное государственное бюджетное учреждение науки Институт физической химии и электрохимии им. А.Н. Фрумкина Российской академии наукРоссияI.M. Sechenov First Moscow State Medical University (Sechenov University); Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of SciencesRussian FederationИнститут общей физики им. А. М. Прохорова Российской академии наук; Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет); Национальный исследовательский ядерный университет «МИФИ»РоссияProkhorov General Physics Institute of the Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University (Sechenov University); Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhIRussian FederationПервый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет); Национальный исследовательский ядерный университет «МИФИ»РоссияI.M. Sechenov First Moscow State Medical University (Sechenov University); Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhIRussian FederationПервый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет)РоссияI.M. Sechenov First Moscow State Medical University (Sechenov University)Russian FederationHuadong Hospital, Fudan UniversityКитайHuadong Hospital, Fudan UniversityChinaФедеральное государственное бюджетное учреждение науки Институт физической химии и электрохимии им. А.Н. Фрумкина Российской академии наук; Институт общей и неорганической химии имени Н.С. Курнакова Российской академии наукРоссияFrumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of SciencesRussian Federation2025171020251432429Copyright © Романишкин И.Д., Меерович И.Г., Бунин Д.А., Скобельцин А.С., Ахлюстина Е.В., Левкин В.В., Харнас С.С., Коган Е.А., Chen Z., Меерович Г.А., Горбунова Ю.Г., Решетов И.В., 20252025Романишкин И.Д., Меерович И.Г., Бунин Д.А., Скобельцин А.С., Ахлюстина Е.В., Левкин В.В., Харнас С.С., Коган Е.А., Chen Z., Меерович Г.А., Горбунова Ю.Г., Решетов И.В.Romanishkin I.D., Meerovich I.G., Bunin D.A., Skobeltsin A.S., Akhlyustina E.V., Levkin V.V., Kharnas S.S., Kogan E.A., Chen Z., Meerovich G.A., Gorbunova Y.G., Reshetov I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/732

Поликатионные фотосенсибилизаторы ранее продемонстрировали высокую эффективность in vitro против клеток рака лёгкого, в том числе против раковых стволовых клеток, при низкой темновой цитотоксичности. Поликатионные фталоцианины имеют высокий квантовый выход фотогенерации синглетного кислорода и фотостабильность. Кроме этого, возможно относительно простое введение различных металлов-комплексообразователей и заместителей во фталоцианиновые макроциклы, что дает возможность варьирования фотофизических характеристик. В данной работе мы изучали фотофизические свойства фотосенсибилизаторов на основе поликатионных производных фталоцианинов с различной химической структурой, обладающих интенсивным поглощением в длинноволновой области (680–690 нм). Исследованные фотосенсибилизаторы проявляют незначительную агрегацию в диапазоне концентраций 1–100 мкМ и демонстрируют очень высокую фототоксичность в исследовании in vitro на клетках карциномы лёгкого A549 (IC50 60–100 нМ для ZnPcChol8 и 100–300 нМ для 4αZnPc4+ и 4αβZnPc4+ в зависимости от дозы света), а также низкую темновую цитотоксичность.

Polycationic photosensitizers have previously demonstrated high in vitro efficacy against lung cancer cells, including cancer stem cells, and low dark cytotoxicity. Polycationic phthalocyanines have high quantum yield of singlet oxygen and photostability. In addition, it is possible to relatively simply introduce different metal-complexing agents and substituents into phthalocyanine macrocycles, which enables varying their photophysical characteristics. In this work, we studied photophysical properties of photosensitizers based on polycationic phthalocyanine derivatives with different chemical structure with strong absorption in the long wavelength region (680–690 nm). The studied photosensitizers exhibit negligible aggregation in the 1–100 μM concentration range and show very high phototoxicity in an in vitro study on A549 lung carcinoma cells (IC50 of 60–100 nM for ZnPcChol8 and 100–300 nM for 4αZnPc4+ and 4αβZnPc4+, depending on the light dose), and low dark cytotoxicity.

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The authors declare that there are no conflicts of interest present.