biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2026-15-2-18-26bioph-791Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИсследование степени фотоокисления липофусцина методами видимой спектроскопии и время-коррелированной флуоресцентной микроскопииInvestigation of lipofuscin photooxidation extent using visible spectroscopy and fluorescence lifetime imaging microscopyМорозовП. В.MorozovP. V.

Москва

Moscow

1pavelmorozov@mail.ruАндреевВ. С.AndreevV. S.

Москва

Moscow

ТокаревМ. А.TokarevM. A.

Москва

Moscow

ЯковлеваM. А.YakovlevaM. A.

Москва

Moscow

КостюковA. А.KostyukovA. A.

Москва

Moscow

ФельдманT. Б.FeldmanT. B.

Москва

Moscow

КузьминВ. А.KuzminV. A.

Москва

Moscow

ОстровскийM. А.OstrovskyM. A.

Москва

Moscow

ГольцманГ. Н.GoltsmanG. N.

Москва

Moscow

Московский Педагогический Государственный Университет; ООО «Сверхпроводниковые нанотехнологииРоссияMoscow Pedagogical State University; LLC “Supconductor Nanotehnology”Russian FederationМосковский Педагогический Государственный Университет; ООО «Сверхпроводниковые нанотехнологии; Национальный Университет Высшая Школа ЭкономикиРоссияMoscow Pedagogical State University; LLC “Supconductor Nanotehnology”; National Research University Higher School of EconomicsRussian FederationМосковский Педагогический Государственный УниверситетРоссияMoscow Pedagogical State UniversityRussian FederationИнститут биохимической физики имени Н.М. Эмануэля РАН; Московский государственный университет имени М.В. ЛомоносоваРоссияN.M. Emanuel Institute of Biochemical Physics RAS; M.V. Lomonosov Moscow State UniversityRussian FederationИнститут биохимической физики имени Н.М. Эмануэля РАНРоссияN.M. Emanuel Institute of Biochemical Physics RASRussian FederationМосковский Педагогический Государственный Университет; Национальный Университет Высшая Школа ЭкономикиРоссияMoscow Pedagogical State University; National Research University Higher School of EconomicsRussian Federation2026240720261521826Copyright © Морозов П.В., Андреев В.С., Токарев М.А., Яковлева M.А., Костюков A.А., Фельдман T.Б., Кузьмин В.А., Островский M.А., Гольцман Г.Н., 20262026Морозов П.В., Андреев В.С., Токарев М.А., Яковлева M.А., Костюков A.А., Фельдман T.Б., Кузьмин В.А., Островский M.А., Гольцман Г.Н.Morozov P.V., Andreev V.S., Tokarev M.A., Yakovleva M.A., Kostyukov A.A., Feldman T.B., Kuzmin V.A., Ostrovsky M.A., Goltsman G.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/791

Исследование посвящено разработке раннего доклинического метода диагностики возрастной макулярной дегенерации с использованием данных время-коррелированной флуоресцентной микроскопии и видимой спектроскопии при анализе кинетик затухания и спектров флуоресценции для липофусциновых гранул. Липофусциновые гранулы были получены из клеток ретинального пигментного эпителия кадаверных глаз человека без признаков патологии. Для изучения кинетики затухания флуоресценции липофусциновых гранул использовали метод возбуждения флуоресценции с усредненной по образцу корреляцией по времени и метод время-разрешенной флуоресцентной визуализированной микроскопии (FLIM) в сочетании со сверхпроводниковым однофотонным детектором. В качестве модели возрастной макулярной дегенерации использовали фотоокисленные гранулы. Сравнительный анализ кинетик затухания и спектров флуоресценции до и после фотоокисления липофусциновых гранул показал достоверные различия характерных времен жизни, а также смещение максимума спектра флуоресценции в коротковолновую область по мере окисления образцов. Полученные данные позволяют сделать вывод о том, что для разработки метода спектрально-кинетического анализа аутофлуоресценции глазного дна необходимо учитывать изменение вклада τ2 и τ3 компонент разложения кинетической кривой затухания флуоресценции τm с наибольшим характерным временем жизни и смещение пика спектра флуоресценции. Такой подход позволит определить диагностические критерии для нормы и патологии при диагностике дегенеративных заболеваний сетчатки и ретинального пигментного эпителия. Анализ полученных данных показал, что метод FLIM, может стать перспективным для разработки раннего доклинического метода диагностики возрастной макулярной дегенерации сетчатки.

The study is devoted to the development of an early preclinical method for the diagnosis of age-related macular degeneration using timecorrelated fluorescence microscopy and visible spectroscopy data for the analysis of fluorescence lifetimes and spectra for lipofuscin granules. Lipofuscin granules were obtained from cells of the retinal pigment epithelium of human cadaveric eyes without signs of pathology. To research the fluorescence lifetimes of lipofuscin granules we used the fluorescence excitation method with time-correlated single photon counting technique and the fluorescence lifetimes imaging microscopy (FLIM) method in combination with a superconducting single-photon detector. Photo-oxidized granules were used as a model of age-related macular degeneration. A comparative analysis of the fluorescence lifetimes and spectra before and after photooxidation of lipofuscin granules showed significant differences in the characteristic lifetimes, as well as a shift of the maximum of the fluorescence spectrum to the short-wavelength region. Analysis of the obtained data confirms the possibility of developing a comprehensive method for the spectral and lifetime characterization of fundus autofluorescence. It is necessary to take into account the changes in the contribution of the τlifetime and the shift in the peak of the fluorescence spectrum. This approach will make it possible to determine diagnostic criteria for norm and 2 and τ3 components of the decomposition of the lifetimes curve of fluorescence decay with the longest characteristic pathology in the diagnosis of degenerative diseases of the retina and retinal pigment epithelium. Analysis of the data obtained showed that the FLIM method might become promising for the development of an early preclinical method for the diagnosis of age-related macular degeneration of the retina.

липофусциновая гранулаFLIMаутофлуоресценция глазного дна (FAF)бисретиноидкинетика затухания флуоресценцииSSPD.lipofuscin granule FLIMfundus autofluorescence (FAF)bisretinoidfluorescence lifetimeSSPDThis work was supported by grant No. 23-65-10005 from the Russian Science Foundation. This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project No. 122041400102-9) and as part of a state assignment to Lomonosov Moscow State UniversityThis work was supported by grant No. 23-65-10005 from the Russian Science Foundation. This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project No. 122041400102-9) and as part of a state assignment to Lomonosov Moscow State UniversityReferencesRózanowska M.B. 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The authors declare that there are no conflicts of interest present.