biophBiomedical PhotonicsBiomedical Photonics2413-9432Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis10.24931/2413-9432-2016-5-3-4-8bioph-96Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИЗУЧЕНИЕ МЕХАНИЗМОВ ФОТОИНДУЦИРОВАННОЙ ГИБЕЛИ НА МОДЕЛИ КЛЕТОК МЕЛАНОМЫ КОЖИTHE STUDY OF MECHANISMS OF PHOTOINDUCED APOPTOSIS IN THE SKIN MALIGNANT MELANOMA CELL MODELГельфондМ. Л.GelfondM. L.mark.gelfond@gmail.comБалдуеваИ. А.BalduevaI. A.БарчукА. С.BarchukA. S.ГафтонГ. И.GaftonG. I.АнисимовВ. В.AnisimovV. V.СемилетоваЮ. В.SemiletovaYu. V.НовикА. В.NovikA. V.МяснянкинМ. Ю.MyasnyankinM. Yu.НехаеваТ. Л.NekhaevaT. L.ДаниловаА. Б.DanilovaA. B.ВоробейчиковЕ. В.VorobeychikovE. V.ВаальА. И.VaaljA. I.ГафтонИ. Г.GaftonI. G.Научно-исследовательский институт онкологии им. Н.Н. Петрова, Санкт-Петербург, Россия Северо-Западный государственный медицинский университет им. И.И. Мечникова, Санкт-Петербург, РоссияРоссияFederal State Institution N.N.Petrov Research Institute of Oncology of Federal Agency of High Technology Medical Assistance, Saint-Petersburg, Russia North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg, RussiaRussian FederationНаучно-исследовательский институт онкологии им. Н.Н. Петрова, Санкт-Петербург, Россия Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова, Санкт-Петербург, РоссияРоссияFederal State Institution N.N.Petrov Research Institute of Oncology of Federal Agency of High Technology Medical Assistance, Saint-Petersburg, Russia Federal State Budgetary Educational Institution of Higher Education Academician I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation, Saint-Petersburg, RussiaRussian FederationНаучно-исследовательский институт онкологии им. Н.Н. Петрова, Санкт-Петербург, РоссияРоссияFederal State Institution N.N.Petrov Research Institute of Oncology of Federal Agency of High Technology Medical Assistance, Saint-Petersburg, RussiaRussian FederationСеверо-Западный государственный медицинский университет им. И.И. Мечникова, Санкт-Петербург, РоссияРоссияNorth-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg, RussiaRussian Federation2016021220165348Copyright © Гельфонд М.Л., Балдуева И.А., Барчук А.С., Гафтон Г.И., Анисимов В.В., Семилетова Ю.В., Новик А.В., Мяснянкин М.Ю., Нехаева Т.Л., Данилова А.Б., Воробейчиков Е.В., Вааль А.И., Гафтон И.Г., 20162016Гельфонд М.Л., Балдуева И.А., Барчук А.С., Гафтон Г.И., Анисимов В.В., Семилетова Ю.В., Новик А.В., Мяснянкин М.Ю., Нехаева Т.Л., Данилова А.Б., Воробейчиков Е.В., Вааль А.И., Гафтон И.Г.Gelfond M.L., Baldueva I.A., Barchuk A.S., Gafton G.I., Anisimov V.V., Semiletova Y.V., Novik A.V., Myasnyankin M.Y., Nekhaeva T.L., Danilova A.B., Vorobeychikov E.V., Vaalj A.I., Gafton I.G.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pdt-journal.com/jour/article/view/96

В статье представлены результаты экспериментального исследования иммунного ответа клеток меланомы кожи человека Mel 226 на фотодинамическое воздействие. Изучен фотоиндуцированный апоптоз клеток меланомы кожи in vitro. Исследования показали, что облучение с препаратом фотодитазин в концентрациях 0,5-2,5 мкг/мл (экспозиция в течение 6 и 10 мин за 30 мин до облучения; параметры облучения: длина волны 662 нм, суммарная доза облучения от 40 до 60 Дж/см2) индуцирует ранний апоптоз. Увеличение времени лазерного облучения достоверно ускоряет переход фотосенсибилизированных опухолевых клеток из ранней фазы апоп-тоза в позднюю.

The results of the experimental study of immune response of human skin malignant melanoma cells Mel 226 on photodynamic exposure are represented in the article. Photoinduced apoptosis of skin malignant melanoma was studied in vitro. The study showed that irradiation with the agent fotoditazin at dose of 0.5–2.5 µg/ml (6 and 10 min exposure 30 min before irradiation; irradiation parameters: wavelength of 662 nm, total light dose from 40 to 60 J/cm2) induced early apoptosis. The increase of the time of laser irradiation significantly accelerates the conversion of photosensitized tumor cells from early to late apoptosis.

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The authors declare that there are no conflicts of interest present.