Photodynamic activity of tetraazachlorin derivate studied in vivo
Abstract
The results of investigation for photodynamic activity of new tetraazachlorin derivate – tetramethyltribenzotetraazachlorin, synthesized in Research Institute of Organic Intermediates and Dyes. The study was performed on female mice of СВА line. The tumor model was transferred solid ascetic sarcoma S-37. The samples of photosensitizer, previously solubilized in 10% aqueou s solution ofCremophor EL, injected to mice intravenously on the 7th day of tumor growth in dose of 1–2 mg/kg. Two hours later the irradiation of sensitized tumor using light emitting diode device in a maximal wavelength of 755 nm (light power density – 50 mW/cm2, maximal total light dose – 300 J/cm2) was performed. The efficacy of photodynamic therapy was assessed by growth inhibition rates in the study group comparing with control group. The study showed that photodynamic therapy with investigated sample in dose of 2 mg/kg and light dose of 300 J/cm2 significantly inhibited the tumor growth (inhibition rate of 70–80% within 20 days), indicating prospectivity of subsequent investigations of tetraazachlorin as photosensitizer for photodynamic therapy of malignant tumors.
About the Authors
V. I. Ivanova-Radkevich
Research Institute of Organic Intermediates and Dyes, Moscow
Russian Federation
Russian Federation
Е. А. Machinskaya
Research Institute of Organic Intermediates and Dyes, Moscow
Russian Federation
Russian Federation
References
1. Странадко Е.Ф. Исторический очерк развития фотодинамической терапии: Обзор // Лазерная медицина. – 2002. – Т. 6., № 1. – С. 4–8.
2. Castano A.P. et al. Mechanisms in photodynamic therapy: part one – photosensitizers, photochemistry and cellular localization // Photodiagnosis and Photodynamic Therapy. – 2004. – № 1. – P. 279–93.
3. Xu D.Y. Research and development of photodynamic therapy photosensitizers in China // Photodiagnosis and Photodynamic Therapy. – 2007. – № 4. – P. 13–25.
4. Иванова-Радкевич В.И., Негримовский В.М., Барканова С.В., Макарова Е.А., Донягина В.Ф., Плетенева Т.В. Биокинетические исследования фотодинамической активности новых фотосенсибилизаторов // Химико-фармацевтический журнал. – 2009. – Т. 43, № 5. – С. 7–10.
5. Иванова-Радкевич В.И., Умнова Л.В., Барканова С.В., Мака- рова Е.А., Лукьянец Е.А. Фотодинамическая активность производного тетраазахлорина // Рос. биотерапевтический журн. – 2008. – T. 7, № 3. – С. 39–41.
6. Барканова С.В., Быстрицкий Г.И., Ворожцов Г.Н. и др. Тетраазахлорины как фотосенсибилизаторы для фотодинамической терапии. Патент РФ 2 278 119 (2006) // Бюлл. Изобр. – 2006. – № 17.
Review
For citations:
Ivanova-Radkevich V.I., Machinskaya Е.А. Photodynamic activity of tetraazachlorin derivate studied in vivo. Photodynamic therapy and photodyagnosis. 2013;2(2):13-18. (In Russ.)
Views:
841
Email this article 