Preview

Biomedical Photonics

Advanced search

Cytological effects in lymph nodes of abdominal lymphodissection zone after intraoperative photodynamic therapy of gastrointestinal cancers

https://doi.org/10.24931/2413-9432-2018-7-4-11-15

Abstract

Cytological studies on lymph nodes of abdominal lymphodissection zone after local intraoperative photodynamic therapy (IOPDT) of gastrointestinal cancers were carried out. As a result of the PDT, the metastatic cells are destroyed, their cytoplasmic membranes and the cytoplasm disappears, leaving behind interphase nuclei ("naked nuclei") (p<0,0001). Cytological confrmation of apoptosis (the presence of apoptotic bodies) in metastatic lymph nodes after IOPDT sessions on the lymph nodes of the abdominal lymphodissection zone is also presented.

About the Authors

V. Yu. Kravtsov
Military Medical Academy of S.M. Kirov, Saint-Petersburg; North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg
Russian Federation


E. S. Pirozhenko
Military Medical Academy of S.M. Kirov, Saint-Petersburg
Russian Federation


K. V. Pavelec
North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg; Saint-Petersburg State Pediatric Medical University, Saint-Petersburg; Mariinsky hospital, St-Petersburg
Russian Federation


M. A. Protchenkov
North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg; Mariinsky hospital, St-Petersburg
Russian Federation


U. A. Drozd
Saint-Petersburg State Pediatric Medical University, Saint-Petersburg
Russian Federation


References

1. Pavelec K.V., Orel V.I., Kravtsov V.Y., Protchencov M.A., Dysin A.V., Drozd U.A., Kuzmina T.I., Kostina U.D., Pavelec M.K., Rusanov D.S., Lacko E.F. Apoptosis in the lymph nodes of the abdominal lymphodissection zone after local intraoperative photodynamic therapy of gastrointestinal cancers, Biomedical Photonics, 2017, vol. 6, no. 3, pp. 39–44. (In Russian)

2. Teiten M.H., Bezdetnaya L., Morliere P., Santus R., Guillemin F. Endoplasmic reticulum and Golgi apparatus are the preferential sites of Foscan localisation in cultured tumour cells, Br J Cancer, 2003, vol. 88, pp. 146–152.

3. Castano A.P., Demidova T.N., Hamblin M.R. Mechanisms in photodynamic therapy: part one—-photosensitizers, photochemistry and cellular localization, Photodiagnosis Photodyn Ther, 2004, vol.1, no. 4, pp. 279–293.

4. Farrahova D.S., YAkovec M.V., Loshchenov V.B., Bolotine L.N., Zorin V.P. Investigation of chlorin photosensitizers distribution in monolayer and spheroid cell cultures, Biomedical Photonics, 2017, vol. 6, no. 2, pp. 4–11. (In Russian)

5. Fontana L.C., Pinto J.G., Pereira A.H.C., Soares C.P., Raniero L.J., Ferreira-Strixino J. Photodithazine photodynamic effect on viability of 9L/lacZ gliosarcoma cell line, Lasers Med Sci, 2017, vol. 32, no. 6, pp. 1245–1252.

6. Pavelets K.V., Kravtsov V.Yu., Protchenkov M.A., Savinov I.P., Vitaleva U.A., Rusanov D.S., Fedorova P.S., Pavelets M.K., Kostina Yu.D. First experience of using intraoperative fluorescent diagnostics and photodynamic therapy in abdominal oncology, Biomedical Photonics, 2015, S. 1, pp. 69–70. (In Russian)

7. Zhou Y.K., Wu W.Z., Zhang L., Yang C.H., Wang Y.P. effect of M007 mediated photodynamic therapy on proliferation of human osteosarcoma MG63 cells in vitro, Sichuan Da Xue Xue Bao Yi Xue Ban, 2012, vol. 43, no. 1, pp. 41–45.

8. Moan J., Berg K. The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen, Photochem Photobiol, 1991, vol. 53, no. 4, pp. 549–553.

9. Kessel D., Luol Y., Deng Y., Chang C.K. The Role of Subcellular Localization in Initiation of Apoptosis by Photodynamic Therapy Photochemistry and Photobiology, 1997, vol. 65, no. 3, pp. 422–426.


Review

For citations:


Kravtsov V.Yu., Pirozhenko E.S., Pavelec K.V., Protchenkov M.A., Drozd U.A. Cytological effects in lymph nodes of abdominal lymphodissection zone after intraoperative photodynamic therapy of gastrointestinal cancers. Biomedical Photonics. 2018;7(4):11-15. https://doi.org/10.24931/2413-9432-2018-7-4-11-15

Views: 1010


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2413-9432 (Print)