Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma
https://doi.org/10.24931/2413-9432-2021-10-4-35-43
Abstract
Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlorin e6 was used for intraoperative fluorescence control at a dose of 1 mg/kg. During the operation, the fluorescence intensity of various areas of the putative tumor tissue was analyzed using the RSS Cam – Endo 1.4.313 software. Tissue samples with various degrees of fluorescence intensity were compared with the results of their histopathological analysis (WHO tumor diagnosis, Ki-67 index, P53, VEGF). Fluorescence was detected in more than half of the cases, but in most cases had a focal character and low fluorescence intensity. The fluorescence intensity directly correlated with the data of histopathological examination of tumor tissues (Ki-67 index (p=0.002), expression of P53 (p=0.0015) and VEGF (p=0.001)). The sensitivity of the method for LGG surgery was 72%, the specificity was 56,7%. Intraoperative fluorescence diagnostics with chlorin e6 can be used in LGG surgery, especially to visualize intratumoral areas with a higher degree of anaplasia.
About the Authors
A. Yu. Rynda
Polenov Neurosurgical Institute – a branch of Almazov National Medical Research Center
Russian Federation
Russian Federation
Saint-Petersburg
V. E. Olyushin
Polenov Neurosurgical Institute – a branch of Almazov National Medical Research Center
Russian Federation
Russian Federation
Saint-Petersburg
D. M. Rostovtsev
Polenov Neurosurgical Institute – a branch of Almazov National Medical Research Center
Russian Federation
Russian Federation
Saint-Petersburg
Y. M. Zabrodskaya
Polenov Neurosurgical Institute – a branch of Almazov National Medical Research Center
Russian Federation
Russian Federation
Saint-Petersburg
G. V. Papayan
Polenov Neurosurgical Institute – a branch of Almazov National Medical Research Center
Russian Federation
Russian Federation
Saint-Petersburg
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Review
For citations:
Rynda A.Yu., Olyushin V.E., Rostovtsev D.M., Zabrodskaya Y.M., Papayan G.V. Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma. Biomedical Photonics. 2021;10(4):35-43. https://doi.org/10.24931/2413-9432-2021-10-4-35-43
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