Сравнение оптико-спектральных характеристик глиобластомы при интраоперационной диагностике и оптической биопсии ex vivo

Сложность интраоперационного определения границ глиальных опухолей обусловлена особенностями их роста вдоль сосудов и нервных волокон с инфильтрацией здорового белого вещества. При этом недостаточно полное удаление опухолевых тканей приводит к рецидивам, а избыточное удаление чревато неврологическими осложнениями. Методы оптической спектроскопии характеризуются высокой скоростью, точностью и неинвазивностью, что обуславливает перспективность их использования для интраоперационной демаркации границ таких опухолей. Спектроскопия флуоресценции и диффузного отражения нашли широкое применение в интраоперационной нейронавигации, главным образом, для обнаружения краев диффузных глиом. При этом в последние годы активно развивается направление ex vivo спектрального анализа образцов опухолей с помощью сочетания различных методов оптической спектроскопии, включающих спектроскопию как упругого, так и неупругого рассеяния. Очевидно, возможности регистрации спектров при интраоперационной работе и на свежих образцах отличаются. В настоящей статье проведено сравнение результатов анализа оптико-спектральных характеристик внутричерепных опухолей при интраоперационной диагностике и анализе ex vivo, а также предложена математическая модель для интерпретации наблюдаемых зависимостей.

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