Study of accumulation of water-soluble asymmetric cationic porphyrins in gram-positive wound infection pathogens during photodynamic inactivation

The paper presents the results of a study on the accumulation of three dierent compounds of water-soluble asymmetric cationic porphyrins by the bacteria S. aureus, S. epidermidis, S. haemolyticus and E. faecalis using ow cytouorimetry and uorescence microscopy. The studied microorganisms were a sample (n=4) of isolates from biomaterial (wound discharge) from patients with wound infections (burn wound, trophic ulcer, infection of the surgical area, etc.). The tested strains showed resistance to 1-7 antibiotics, two strains were carriers of the mecA gene. Porphyrins containing heterocyclic fragments (benzoxazole, N-methyl benzimidazole, and benzothiazole residues) on the periphery of the porphyrin cycle can accumulate in bacterial cells to varying degrees: porphyrin with N-methyl benzimidazole penetrates bacteria to a greater extent, and the uorescence signal is most intense for S. aureus and E. faecalis after incubation with this species. connections. There is some heterogeneity in the bacterial cell population with respect to the ability to accumulate porphyrins, and the presence of bacterial lysis has been proven. S aureus after incubation with S-porphyrin and subsequent photodynamic inactivation under the inuence of light. The data obtained determine the prospects for further study of compounds and determination of their bactericidal potential.

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