Results of comparative experimental and morphological study of activity on corneal neovascularization in rabbit’s eye of photodynamic therapy with Photosense and antiangiogenic therapy with Avastin (bevacizumab) alone, and in its combination are represented. The treatment was performed in regimens: 1) photodynamic therapy with Photosense (single intravenous injection in dose of 0.3 mg/kg 72 h prior to laser irradiation, power density of irradiation 500 mW/cm², wavelength 675 nm); 2) drug antiangiogenic therapy with Avastin (single subconjuctival introduction at dose of 1.25 mg); 3) photodynamic therapy with Photosense (in described regimen) in combination with subconjuctival introduction of Avastin at dose of 1.25 mg immediately prior to irradiation. Combined photodynamic therapy with antiangiogenic therapy allows to obtain almost complete occlusion in all regions of corneal neovascular bed with shorter time interval comparing with those for monoregimens. In case of blood flow retention in great vessels courses of combined modality treatment may be repeated. The absence of side-effects on surrounding tissues and the recovery of optical characteristics of cornea may allow to use this upcoming method in clinical practice.

The results of photodynamic therapy (PDT) in patients with skin cancer are represented. The treatment was performed in 283 patients with basal cell skin cancer (BCSC) and also in 51 patients with squamous cell skin cancer (SCSC). For PDT the photosensitizer Photolon at dose of 1.1–1.6 mg/kg body weight was used. The light irradiation dose delivered to tumor was from 100 to 600 J/cm² by one or several fields according to type and size of tumor lesion. After PDT for BCSC the complete regression of tumor lesions was in 96.8% of cases, partial regression – in 3.2%. For PDT of recurrent tumors the complete regression was observed in 76.4% of cases, partial regression – in 23.6%. After PDT in patients with primary SCSC the complete regression was achieved in 91.7% of patients and partial regression – in 8.3%. For treatment of recurrent SCCS the complete regression was observed only in 59.3% of cases, partial – in 33.3%, no response – in 7.4%. For follow-up period from 6 months to 5 years recurrent SCSC was diagnosed in 31.2% of patients. The results shows that the application of PDT allows to perform effective treatment of basal and squamous cell skin cancer for unfavorable localization of tumor and in case of limitations for surgical treatment and/or radiotherapy and the efficacy of treatment depends on stage of disease. clinical and morphological pattern of process and previous treatment modality.

Results of photodynamic therapy in 96 patients with primary and recurrent basal cell skin cancer of ENT-organs are represented. For photodynamic therapy the Russian-made photosensitizer Photoditazine at dose of 0.6–1.4 mg/kg was used. Parameters were selected taking into account type and extent of tumor and were as follows: output power – 0.1–3.0 W, power density – 0.1–1.3 W/cm2, light dose – 100–400 J/cm2. The studies showed high efficacy of treatment for primary and recurrent basal cell skin cancer of nose, ear and external auditory canal – from 87.5 to 94.7% of complete regression. Examples of efficacy of the method are represented in the article. High efficacy and good cosmetic effects allowed to make a conclusion about perspectivity of photodynamic therapy for recurrent basal cell skin cancer of ENT-organs.

Results of diagnosis and treatment in 32 patients with lower lip cancer using photodynamic therapy (PDT) with photosensitizer Photosense are represented. 15 patients had stage I of disease, in 12 – stage II and in 5 – stage III. Photosensitizer Photosense was administered at dose of 0.5 mg/kg body weight in 20 patients and at dose of 0.8 mg/kg – in 12 patients as single intravenous infusion. During the treatment the fluorescent diagnosis (FD) was performed. The average power of diagnostic laser irradiation was 2 mW, light dose on tissue surface was < 1 J/cm². Power density of therapeutic laser irradiation accounted for 150–300 mW/cm², light dose of one session was 200-300 J/cm². First session of PDT was performed 24 h after drug injection, the interval between following sessions accounted for 24 h. The number of session varied from 3 to 5. The results of FD showed the increase of fluorescent intensity in the center of tumor from 14.9 up to 150.8 r.u. 24 h after drug injection, this parameter in the normal mucosa increased from 13,4 to 88,3 r.u. The side-effects of PDT with Photosense included pain syndrome, which was managed by non-opioid analgesics in all 10 patients. The signs of phototoxycity were observed in 9 patients. Two months after PDT 22 (68.7%) patients had complete regression of tumor lesion, the response in 8 (25%) patients was defined as partial, 2 (6.3%) patients had stabilization of tumor growth.

The technique of fluorescence hysteroscopy with Alasens includes visual assessment of fluorescence of Alasens-induced protoporphyrin IX and local fluorescence spectroscopy. The technique allows to improve the efficacy of early diagnosis for endometrial pathology including early endometrial cancer, to assess definitely an extent of pre-tumor and tumor process. The sensitivity of fluorescence hysteroscopy accounts for 100%, the specificity – 98%.

A case of successful photodynamic therapy in patient of 86 y.o. with diagnosis: angiosarcoma of right temporal-parietal region stage IIA (Т_2вN₀M₀) is reported. The tumor was as soft tissue round shape lesion with tuberous contours 3.4х3.4х1.1 cm in size, located in subcutaneous tissue in right parietal region with no scull bone invasion. The patient was refused to surgical treatment with general anesthesia due to severe cardiovascular co-morbidity. The patient underwent a course of photodynamic therapy with Photolon. The photosensitizer was intravenousely introduced for 3 h before irradiation at dose of 1 mg/kg body weight. The parameters of irradiation were as follows: output power – 0.8 W, light dose – 150 J/cm², 4 irradiation fields 2.5 cm in diameter. During the irradiation there were moderate pain which did not require drug management. After PDT complete regression of the tumor was achieved. For nowadays (11 months after treatment) the patient is observed with no recurrence. The reported case shows that photodynamic therapy may be successfully used for alternative treatment of soft tissue angiosarcoma in patients with no ability for surgical treatment.

A case of multiple course of photodynamic therapy (PDT) in patient with gastric cancer T₁N₀M₀. Morpholological diagnosis in this patient was signet ring cell cancer. For 8 years the patient underwent endoscopic organ-sparing treatment: PDT with Photohem (17 courses), electrocoagulation of tumor (3 sessions). The drug Photohem was introduced intravenously at dose of 3.0 mg/kg body weight for 48 h before PDT. The treatment result was only partial regression of gastric tumor, the maximal follow-up period with no endoscopic and morphological signs of tumor growth accounted for 8 months. However besides incomplete removal of gastric tumor and morphological type, for check-up examination 8 years after the onset of endoscopic treatment there were no features of regional and distant metastases according to chest and abdominal CT and US.